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CK1alpha and IRF4 are essential and independent effectors of immunomodulatory drugs in primary effusion lymphoma.
Patil A, Manzano M, Gottwein E
Blood. 2018 Aug 9;132(6):577-586. doi: 10.1182/blood-2018-01-828418. Epub 2018 Jun 28. PubMed Article

Plasmids from Article

ID Plasmid Purpose
123318pLCE-P2A-PuroLentiviral vector for expression of eGFP-P2A-Puro cassette from a CMV promoter
123319pLC-Flag-CSNK1A1 WT-PuroLentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoter
123320pLC-Flag-CSNK1A1 G40N-PuroLentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoter
123321pLC-Flag-CSNK1A1 G40N-P2A-BlastLentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Blast casette from a CMV promoter
123322pLC-ZsGreen-P2A-BlastLentiviral vector for expression of ZsGreen-P2A-Blast cassette from a CMV promoter
123323pCW-IRF4-PuroLentiviral vector for expression of IRF4 under a Doxycycline inducible promoter
123324pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh1Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert
123325pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh2Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert
123326pZIP-hCMV-ZsGreen-P2A-Puro-IKZF3 sh1Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert
123327pZIP-hCMV-ZSGreen-P2A-Puro-IKZF3 sh2Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert
123328pZIP-hCMV-ZsGreen-P2A-Puro-NT4Lentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Antibodies from Article