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Ulrich Elling Lab: CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci.

Chylinski K, Hubmann M, Hanna RE, Yanchus C, Michlits G, Uijttewaal ECH, Doench J, Schramek D, Elling U
Nat Commun. 2019 Nov 29;10(1):5454. doi: 10.1038/s41467-019-13403-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
217883p204-U6-Switch-ONRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR
217884p205-5'-Switch-ONRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR
217885p206-Switch-ONRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR
217886p207-Switch-ON (FRT)Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR
217888p213-SWITCH-OFFRetroviral Switch-OFF vector for sgRNA expression; U6-BbsIx2-SWITCH-OFF-scaffold; neoR
217889p223-Pulse-SwitchLentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiR
217890p931-SWITCH-OVER insert: EF1a-Blastiinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6
217891p944-SWITCH-OVER insert: EF1a-Puroinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6
217892p1071-Lenti-Single loxPLentiviral vector, entry vector for Lentiviral Switch-OVER

Antibodies from Article