CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci.
Chylinski K, Hubmann M, Hanna RE, Yanchus C, Michlits G, Uijttewaal ECH, Doench J, Schramek D, Elling U
Nat Commun. 2019 Nov 29;10(1):5454. doi: 10.1038/s41467-019-13403-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217883 | p204-U6-Switch-ON | Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR |
| 217884 | p205-5'-Switch-ON | Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR |
| 217885 | p206-Switch-ON | Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR |
| 217886 | p207-Switch-ON (FRT) | Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR |
| 217888 | p213-SWITCH-OFF | Retroviral Switch-OFF vector for sgRNA expression; U6-BbsIx2-SWITCH-OFF-scaffold; neoR |
| 217889 | p223-Pulse-Switch | Lentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiR |
| 217890 | p931-SWITCH-OVER insert: EF1a-Blasti | insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6 |
| 217891 | p944-SWITCH-OVER insert: EF1a-Puro | insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6 |
| 217892 | p1071-Lenti-Single loxP | Lentiviral vector, entry vector for Lentiviral Switch-OVER |