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pLenti-mCherry-Mango II x 24
(Plasmid #127587)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127587 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti/pCW
  • Backbone size w/o insert (bp) 7767
  • Total vector size (bp) 9693
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-Mango II x24
  • Species
    Synthetic
  • Insert Size (bp)
    1926
  • Promoter pTRE Tet ON
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer mCherry_F
  • 3′ sequencing primer Puro_R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-mCherry-Mango II x 24 was a gift from David Rueda (Addgene plasmid # 127587 ; http://n2t.net/addgene:127587 ; RRID:Addgene_127587)
  • For your References section:

    Live cell imaging of single RNA molecules with fluorogenic Mango II arrays. Cawte AD, Unrau PJ, Rueda DS. Nat Commun. 2020 Mar 9;11(1):1283. doi: 10.1038/s41467-020-14932-7. 10.1038/s41467-020-14932-7 PubMed 32152311