236,069 results

1. pBBR1MCS-2

   Plasmid

   #85168

   Purpose

   Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

   Depositor

   Kenneth Peterson

   Article

   Kovach et al Gene. 1995 Dec 1;166(1):175-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Constitutive (derepressed P-lac)

   Available Since

   Nov. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pBBR1MCS-2

   Plasmid

   #85168

   Purpose

   Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

   Depositor

   Kenneth Peterson

   Article

   Kovach et al Gene. 1995 Dec 1;166(1):175-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Constitutive (derepressed P-lac)

   Available Since

   Nov. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (AAV1)

   Viral Prep

   #100834-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (#100834). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 plasmid DNA. CamKII-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CaMKII

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1-a and hU6

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1-a and hU6

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCF159_BRL

   Plasmid

   #225962

   Purpose

   CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

   Insert

   CMV-Intron-BRL (env protein)
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCas9-Blast

   Plasmid

   #52962

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Lentiviral Prep

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMDLg/pRRE

    Plasmid

    #12251

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    HIV-1 GAG/POL
    

    Use

    Lentiviral; Packaging

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDLg/pRRE

    Plasmid

    #12251

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    HIV-1 GAG/POL
    

    Use

    Lentiviral; Packaging

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-VSV-G

    Plasmid

    #8454

    Purpose

    Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).

    Depositor

    Bob Weinberg

    Article

    Stewart et al RNA 2003 Apr;9(4):493-501.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-VSV-G

    Plasmid

    #8454

    Purpose

    Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).

    Depositor

    Bob Weinberg

    Article

    Stewart et al RNA 2003 Apr;9(4):493-501.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-NT*-HRV3CP

    Plasmid

    #162795

    Purpose

    Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

    Depositor

    Gottfried Otting

    Article

    Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

    Insert

    Human rhinovirus 14 3C protease
    

    Tags

    NT* solubility tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV.Syn.GCaMP6f.WPRE.SV40 (AAV1)

    Viral Prep

    #100837-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.Syn.GCaMP6f.WPRE.SV40 (#100837). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pLVX-M-puro

    Plasmid

    #125839

    Purpose

    modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAG-tdTomato (codon diversified) (AAV8)

    Viral Prep

    #59462-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    tdTomato (codon diversified)

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-G-Flamp2

    Plasmid

    #192782

    Purpose

    green biosensor for cAMP in mammalian cells

    Depositor

    Jun Chu

    Article

    Liu et al Front Pharmacol. 2022 May 12;13:902290.

    Insert

    G-Flamp2
    

    Tags

    His tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-tdTomato (codon diversified) (AAV Retrograde)

    Viral Prep

    #59462-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. tdTomato expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    tdTomato (codon diversified)

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only