179,378 results
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Plasmid#74223PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tagDepositorTypeEmpty backboneTagsMyc and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pXR001: EF1a-CasRx-2A-EGFP
Plasmid#109049PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targetingDepositorInsertCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianPromoterEF1AAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-GCaMP6f
Plasmid#40755PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6f
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-mCherry
Plasmid#36084Purpose3rd generation lentiviral plasmid expressing mCherryDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV9)
Viral prep#44362-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-KO
Plasmid#17603PurposeMammalian expression of HA tagged ubiquitin carrying zero lysines (K0)DepositorPublicationInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationKO: all lysines mutated to argininesAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV9)
Viral prep#44361-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha-pcDNA3
Plasmid#18949DepositorPublicationInsertHypoxia inducible factor 1 alpha (HIF1A Human)
TagsHA-tagExpressionMammalianMutationwt cDNAAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV5)
Viral prep#20298-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCas9_D10A
Plasmid#41816PurposeExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineeringDepositorInsertCas9_D10A
UseCRISPRExpressionMammalianMutationhuman codon-optimized, D10A nickasePromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-EGFP (AAV Retrograde)
Viral prep#50465-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. Synapsin-driven EGFP-expression control. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.DepositorPromoterSynTagsEGFPAvailable sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 hygro
Plasmid#24150Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.DepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianPromoterU6 for shRNAAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DR274
Plasmid#42250Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcriptionDepositorTypeEmpty backboneUseCRISPR; Zebrafish expressionExpressionWormPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV2)
Viral prep#44362-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SV40 LT
Plasmid#13970DepositorPublicationAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCas9-Blast (Lentiviral Prep)
Viral prep#52962-LVPurposeReady-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.DepositorPromoterEFS-NSAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV rtTA3 Blast (w756-1)
Plasmid#26429Purpose3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, BlastDepositorInsertTetracycline repressor A3 mutant
UseLentiviralExpressionMammalianAvailable sinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
H2B-mCherry
Plasmid#20972PurposeH2B fused to mCherry for chromatin visualizationDepositorAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-GFP(1-10)
Plasmid#70219PurposeExpresses GFP(1-10) in mammalian cellsDepositorInsertGFP(1-10)
ExpressionMammalianAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-dCas9-p300 Core
Plasmid#61357Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by