pXR001: EF1a-CasRx-2A-EGFP Citations (34)
Originally described in: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033. PubMed Journal
Articles Citing pXR001: EF1a-CasRx-2A-EGFP
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| CRISPR-Cas13d mediates robust RNA virus interference in plants. Mahas A, Aman R, Mahfouz M. Genome Biol. 2019 Dec 2;20(1):263. doi: 10.1186/s13059-019-1881-2. PubMed |
Massively parallel Cas13 screens reveal principles for guide RNA design.
Wessels HH, Mendez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana NE.
Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
PubMed
Associated Plasmids
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CRISPR-assisted detection of RNA-protein interactions in living cells.
Yi W, Li J, Zhu X, Wang X, Fan L, Sun W, Liao L, Zhang J, Li X, Ye J, Chen F, Taipale J, Chan KM, Zhang L, Yan J.
Nat Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 10.1038/s41592-020-0866-0.
PubMed
Associated Plasmids |
| Silencing of lncRNA MIR497HG via CRISPR/Cas13d Induces Bladder Cancer Progression Through Promoting the Crosstalk Between Hippo/Yap and TGF-beta/Smad Signaling. Zhuang C, Liu Y, Fu S, Yuan C, Luo J, Huang X, Yang W, Xie W, Zhuang C. Front Mol Biosci. 2020 Dec 9;7:616768. doi: 10.3389/fmolb.2020.616768. eCollection 2020. PubMed |
| Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs. Zhang Y, Nguyen TM, Zhang XO, Wang L, Phan T, Clohessy JG, Pandolfi PP. Genome Biol. 2021 Jan 21;22(1):41. doi: 10.1186/s13059-021-02263-9. PubMed |
| Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro. Zhuang C, Zhuang C, Zhou Q, Huang X, Gui Y, Lai Y, Yang S. Front Mol Biosci. 2021 Mar 19;8:646412. doi: 10.3389/fmolb.2021.646412. eCollection 2021. PubMed |
| Potent programmable antiviral against dengue virus in primary human cells by Cas13b RNP with short spacer and delivery by VLP. Singsuksawat E, Onnome S, Posiri P, Suphatrakul A, Srisuk N, Nantachokchawapan R, Praneechit H, Sae-Kow C, Chidpratum P, Sa-Ngiamsuntorn K, Hongeng S, Avirutnan P, Duangchinda T, Siridechadilok B. Mol Ther Methods Clin Dev. 2021 Jun 11;21:729-740. doi: 10.1016/j.omtm.2021.04.014. Epub 2021 May 1. PubMed |
| In vivo partial reprogramming of myofibers promotes muscle regeneration by remodeling the stem cell niche. Wang C, Rabadan Ros R, Martinez-Redondo P, Ma Z, Shi L, Xue Y, Guillen-Guillen I, Huang L, Hishida T, Liao HK, Nunez Delicado E, Rodriguez Esteban C, Guillen-Garcia P, Reddy P, Izpisua Belmonte JC. Nat Commun. 2021 May 25;12(1):3094. doi: 10.1038/s41467-021-23353-z. PubMed |
| CRISPR/Cas13d-mediated efficient KDM5B mRNA knockdown in porcine somatic cells and parthenogenetic embryos. Bi D, Yao J, Wang Y, Qin G, Zhang Y, Wang Y, Zhao J. Reproduction. 2021 Jul 8;162(2):149-160. doi: 10.1530/REP-21-0053. PubMed |
Methods for in vitro CRISPR/CasRx-Mediated RNA Editing.
Chuang YF, Wang PY, Kumar S, Lama S, Lin FL, Liu GS.
Front Cell Dev Biol. 2021 Jun 11;9:667879. doi: 10.3389/fcell.2021.667879. eCollection 2021.
PubMed
Associated Plasmids |
| The Bacterial Enzyme RfxCas13d Is Less Neurotoxic Than PspCas13b and Could Be a Promising RNA Editing and Interference Tool in the Nervous System. Wu QW, Kapfhammer JP. Brain Sci. 2021 Aug 9;11(8):1054. doi: 10.3390/brainsci11081054. PubMed |
| Efficient Inhibition of HIV Using CRISPR/Cas13d Nuclease System. Nguyen H, Wilson H, Jayakumar S, Kulkarni V, Kulkarni S. Viruses. 2021 Sep 16;13(9). pii: v13091850. doi: 10.3390/v13091850. PubMed |
| Bile Acid-Mediated Activation of Brown Fat Protects From Alcohol-Induced Steatosis and Liver Injury in Mice. Fan M, Wang Y, Jin L, Fang Z, Peng J, Tu J, Liu Y, Zhang E, Xu S, Liu X, Huo Y, Sun Z, Chao X, Ding WX, Yan Q, Huang W. Cell Mol Gastroenterol Hepatol. 2022;13(3):809-826. doi: 10.1016/j.jcmgh.2021.12.001. Epub 2021 Dec 9. PubMed |
| LncRNA SOX1-OT V1 acts as a decoy of HDAC10 to promote SOX1-dependent hESC neuronal differentiation. Xi J, Xu Y, Guo Z, Li J, Wu Y, Sun Q, Wang Y, Chen M, Zhu S, Bian S, Kang J. EMBO Rep. 2022 Feb 3;23(2):e53015. doi: 10.15252/embr.202153015. Epub 2021 Dec 20. PubMed |
| Targeted gene silencing in the nervous system with CRISPR-Cas13. Powell JE, Lim CKW, Krishnan R, McCallister TX, Saporito-Magrina C, Zeballos MA, McPheron GD, Gaj T. Sci Adv. 2022 Jan 21;8(3):eabk2485. doi: 10.1126/sciadv.abk2485. Epub 2022 Jan 19. PubMed |
| CRISPR/CasRx Proof-of-Concept for RNA Degradation: A Future Tool against RNA Viruses? Perez-SanJose D, de la Fuente MA, Serna Perez J, Simarro M, Eiros Bouza JM, Sanz-Munoz I. Pharmaceuticals (Basel). 2021 Dec 27;15(1):32. doi: 10.3390/ph15010032. PubMed |
CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells.
Ai Y, Liang D, Wilusz JE.
Nucleic Acids Res. 2022 Mar 4. pii: 6542487. doi: 10.1093/nar/gkac159.
PubMed
Associated Plasmids |
| Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms. Tian S, Liu Y, Appleton E, Wang H, Church GM, Dong M. Cell Rep. 2022 Mar 8;38(10):110476. doi: 10.1016/j.celrep.2022.110476. PubMed |
| Expression of Circ_Satb1 Is Decreased in Mesial Temporal Lobe Epilepsy and Regulates Dendritic Spine Morphology. Gomes-Duarte A, Veno MT, de Wit M, Senthilkumar K, Broekhoven MH, van den Herik J, Heeres FR, van Rossum D, Rybiczka-Tesulov M, Legnini I, van Rijen PC, van Eijsden P, Gosselaar PH, Rajewsky N, Kjems J, Vangoor VR, Pasterkamp RJ. Front Mol Neurosci. 2022 Mar 3;15:832133. doi: 10.3389/fnmol.2022.832133. eCollection 2022. PubMed |
| ADARs act as potent regulators of circular transcriptome in cancer. Shen H, An O, Ren X, Song Y, Tang SJ, Ke XY, Han J, Tay DJT, Ng VHE, Molias FB, Pitcheshwar P, Leong KW, Tan KK, Yang H, Chen L. Nat Commun. 2022 Mar 21;13(1):1508. doi: 10.1038/s41467-022-29138-2. PubMed |
| PERSIST platform provides programmable RNA regulation using CRISPR endoRNases. DiAndreth B, Wauford N, Hu E, Palacios S, Weiss R. Nat Commun. 2022 May 11;13(1):2582. doi: 10.1038/s41467-022-30172-3. PubMed |
A cell-cycle-dependent GARP-like transcriptional repressor regulates the initiation of differentiation in Giardia lamblia.
Shih HW, Alas GCM, Paredez AR.
Proc Natl Acad Sci U S A. 2022 May 31;119(22):e2204402119. doi: 10.1073/pnas.2204402119. Epub 2022 May 25.
PubMed
Associated Plasmids |
| SAFA facilitates chromatin opening of immune genes through interacting with anti-viral host RNAs. Cao L, Luo Y, Guo X, Liu S, Li S, Li J, Zhang Z, Zhao Y, Zhang Q, Gao F, Ji X, Gao X, Li Y, You F. PLoS Pathog. 2022 Jun 3;18(6):e1010599. doi: 10.1371/journal.ppat.1010599. eCollection 2022 Jun. PubMed |
| Diagnostics of Infections Produced by the Plant Viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13. Marques MC, Sanchez-Vicente J, Ruiz R, Montagud-Martinez R, Marquez-Costa R, Gomez G, Carbonell A, Daros JA, Rodrigo G. ACS Synth Biol. 2022 Jul 15;11(7):2384-2393. doi: 10.1021/acssynbio.2c00090. Epub 2022 Jul 6. PubMed |
| Negative autoregulation mitigates collateral RNase activity of repeat-targeting CRISPR-Cas13d in mammalian cells. Kelley CP, Haerle MC, Wang ET. Cell Rep. 2022 Aug 16;40(7):111226. doi: 10.1016/j.celrep.2022.111226. PubMed |
| Optimization of specific RNA knockdown in mammalian cells with CRISPR-Cas13. Burris BJD, Molina Vargas AM, Park BJ, O'Connell MR. Methods. 2022 Oct;206:58-68. doi: 10.1016/j.ymeth.2022.08.007. Epub 2022 Aug 17. PubMed |
Programmable RNA sensing for cell monitoring and manipulation.
Qian Y, Li J, Zhao S, Matthews EA, Adoff M, Zhong W, An X, Yeo M, Park C, Yang X, Wang BS, Southwell DG, Huang ZJ.
Nature. 2022 Oct;610(7933):713-721. doi: 10.1038/s41586-022-05280-1. Epub 2022 Oct 5.
PubMed
Associated Plasmids |
| Polyvalent guide RNAs for CRISPR antivirals. Bagchi R, Tinker-Kulberg R, Salehin M, Supakar T, Chamberlain S, Ligaba-Osena A, Josephs EA. iScience. 2022 Oct 13;25(11):105333. doi: 10.1016/j.isci.2022.105333. eCollection 2022 Nov 18. PubMed |
| Enhanced RNA knockdown efficiency with engineered fusion guide RNAs that function with both CRISPR-CasRx and hammerhead ribozyme. Zhan Y, Cao C, Li A, Mei H, Liu Y. Genome Biol. 2023 Jan 17;24(1):9. doi: 10.1186/s13059-023-02852-w. PubMed |
| Collateral activity of the CRISPR/RfxCas13d system in human cells. Shi P, Murphy MR, Aparicio AO, Kesner JS, Fang Z, Chen Z, Trehan A, Guo Y, Wu X. Commun Biol. 2023 Mar 28;6(1):334. doi: 10.1038/s42003-023-04708-2. PubMed |
| Mitigating a TDP-43 proteinopathy using RNA-targeting CRISPR effector proteins. Zeballos C MA, Moore HJ, Powell JE, Smith TJ, Zhang S, Gaj T. bioRxiv. 2023 Apr 8:2023.04.07.536072. doi: 10.1101/2023.04.07.536072. Preprint. PubMed |
| Engineered circular guide RNAs boost CRISPR/Cas12a- and CRISPR/Cas13d-based DNA and RNA editing. Zhang X, Wang X, Lv J, Huang H, Wang J, Zhuo M, Tan Z, Huang G, Liu J, Liu Y, Li M, Lin Q, Li L, Ma S, Huang T, Lin Y, Zhao X, Rong Z. Genome Biol. 2023 Jun 23;24(1):145. doi: 10.1186/s13059-023-02992-z. PubMed |
| AAV-CRISPR-Cas13 eliminates human enterovirus and prevents death of infected mice. Keng CT, Yogarajah T, Lee RCH, Muhammad IBH, Chia BS, Vasandani SR, Lim DS, Guo K, Wong YH, Mok CK, Chu JJH, Chew WL. EBioMedicine. 2023 Jul;93:104682. doi: 10.1016/j.ebiom.2023.104682. Epub 2023 Jun 28. PubMed |
| CRISPR-based resistance to grapevine virus A. Spencer KP, Burger JT, Campa M. Front Plant Sci. 2023 Dec 4;14:1296251. doi: 10.3389/fpls.2023.1296251. eCollection 2023. PubMed |
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