Light-induced expression of gRNA allows for optogenetic gene editing of T lymphocytes in vivo.

Pulgarin DV, Pelo N, Ferrandiz L, Trselic T, Nyberg WA, Bowlin G, Espinosa A
Nucleic Acids Res. 2025 Mar 20;53(6):gkaf213. doi: 10.1093/nar/gkaf213. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
220497	pMSCV-BLU-VIPR	Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).
220498	pBLU-VIPR	Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).