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Methods for in vitro CRISPR/CasRx-Mediated RNA Editing.
Chuang YF, Wang PY, Kumar S, Lama S, Lin FL, Liu GS
Front Cell Dev Biol. 2021 Jun 11;9:667879. doi: 10.3389/fcell.2021.667879. eCollection 2021.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
166867pM115: CasRx control presgRNA U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.
166868pM116: CasRx VEGFA presgRNAU6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.
166869pM117: VEGFA array presgRNAU6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.
166870pM122: pAAV-EFS-CasRxAAV vector for expressing CasRx
166871pM123: pAAV-EFS-CasRx-control presgRNAAAV vector for expressing CasRx and control presgRNA for RNA-editing
166872pM124: pAAV-EFS-CasRx-VEGFA presgRNAAAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editing
166873pM125: pAAV-EFS-CasRx-VEGFA array presgRNAAAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing

Antibodies from Article