Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pM125: pAAV-EFS-CasRx-VEGFA array presgRNA
(Plasmid #166873)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166873 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    Cell Biolabs, Inc.
  • Backbone size w/o insert (bp) 3315
  • Total vector size (bp) 6822
  • Modifications to backbone
    1) Remove original sequences (CMV promoter, human b-globin intron, MCS, and hGH polyA signal) between 2 ITRs. 2) Insert EFS promoter/SV40 polyA signal.
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    U6-VEGFA array presgRNA
  • Species
  • Insert Size (bp)
  • Promoter U6

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Alt name
  • Species
    Ruminococcus flavefaciens XPD3002
  • Insert Size (bp)
  • Promoter EFS
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • NLS (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pM125: pAAV-EFS-CasRx-VEGFA array presgRNA was a gift from Guei-Sheung Liu (Addgene plasmid # 166873 ; ; RRID:Addgene_166873)
  • For your References section:

    Methods for in vitro CRISPR/CasRx-Mediated RNA Editing. Chuang YF, Wang PY, Kumar S, Lama S, Lin FL, Liu GS. Front Cell Dev Biol. 2021 Jun 11;9:667879. doi: 10.3389/fcell.2021.667879. eCollection 2021. 10.3389/fcell.2021.667879 PubMed 34178991