PB_EF1a-DjCas13d-msfGFP-Blast
(Plasmid
#226008)
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PurposeDjCas13d protein for RNA targeting in piggyBac backbone
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Depositing Lab
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Depositing OrganizationArc Institute
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 226008 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 226008-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 226008-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneCustom
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Vector typeMammalian Expression, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDjCas13d RNA-targeting nuclease
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Tag
/ Fusion Protein
- msfGFP (C terminal on backbone)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This does not include a guideRNA (see separate lenti plasmid: pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI, Addgene #226009) and needs to be co-transfected with a plasmid encoding the Piggybac transposase for insertion into the genome.
DNA (Catalog # 226008-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 226008-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB_EF1a-DjCas13d-msfGFP-Blast was a gift from Silvana Konermann (Addgene plasmid # 226008 ; http://n2t.net/addgene:226008 ; RRID:Addgene_226008) -
For your References section:
Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA targeting. Wei J, Lotfy P, Faizi K, Baungaard S, Gibson E, Wang E, Slabodkin H, Kinnaman E, Chandrasekaran S, Kitano H, Durrant MG, Duffy CV, Pawluk A, Hsu PD, Konermann S. Cell Syst. 2023 Dec 20;14(12):1087-1102.e13. doi: 10.1016/j.cels.2023.11.006. Epub 2023 Dec 12. 10.1016/j.cels.2023.11.006 PubMed 38091991
Map uploaded by the depositor.
