pLKO.1 hygro
(Plasmid #24150)

• Purpose: (Empty Backbone) 3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
• Depositing Lab: Bob Weinberg
• Depositing Organization: Whitehead Institute for Biomedical Research
  Located in United States of America
• Publication: empty vector (unpublished)
• Citations: 76 Articles

Backbone

• Vector backbone: pLKO.1 - TRC cloning vector (no stuffer)
• Backbone manufacturer: David Root
• Backbone size (bp): 7500
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Promoter: U6 for shRNA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LKO1_5
• 3′ sequencing primer: SV40pA-R

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Reference:
  • Addgene's pLKO.1 protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is similar to the pLKO.1-TRC cloning vector (www.addgene.org/10878) except the stuffer between AgeI and EcoRI is only about 24 bp (instead of 1.7kb) and the puro gene has been replaced by hygro resistance.