PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1771||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Alt nametelomerase reverse transcriptase
SpeciesH. sapiens (human)
Insert Size (bp)3500
Entrez GeneTERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE-3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that there is a point mutation at base 1547, from A to G. This changes a glycine to an aspartate. Thus far it appears to behave like wild-type, but extensive biochemistry has not been performed.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABE-puro-hTERT was a gift from Bob Weinberg (Addgene plasmid # 1771 ; http://n2t.net/addgene:1771 ; RRID:Addgene_1771)
For your References section:Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Counter CM, Hahn WC, Wei W, Caddle SD, Beijersbergen RL, Lansdorp PM, Sedivy JM, Weinberg RA. Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8. 10.1073/pnas.95.25.14723 PubMed 9843956