lenti sgRNA(MS2)_zeo backbone Citations (101)
Originally described in: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F Nature. 2014 Dec 10. doi: 10.1038/nature14136. PubMed Journal
Articles Citing lenti sgRNA(MS2)_zeo backbone
| Articles |
|---|
| Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease. Dahlman JE, Abudayyeh OO, Joung J, Gootenberg JS, Zhang F, Konermann S. Nat Biotechnol. 2015 Oct 5;33(11):1159-1161. doi: 10.1038/nbt.3390. PubMed |
| CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs. Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. Sci Rep. 2015 Nov 5;5:16277. doi: 10.1038/srep16277. PubMed |
| CRISPR-mediated Activation of Latent HIV-1 Expression. Limsirichai P, Gaj T, Schaffer DV. Mol Ther. 2015 Nov 26. doi: 10.1038/mt.2015.213. PubMed |
| Specific induction of endogenous viral restriction factors using CRISPR/Cas-derived transcriptional activators. Bogerd HP, Kornepati AV, Marshall JB, Kennedy EM, Cullen BR. Proc Natl Acad Sci U S A. 2015 Dec 29;112(52):E7249-56. doi: 10.1073/pnas.1516305112. Epub 2015 Dec 14. PubMed |
Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC.
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
PubMed
Associated Plasmids |
| Empower multiplex cell and tissue-specific CRISPR-mediated gene manipulation with self-cleaving ribozymes and tRNA. Xu L, Zhao L, Gao Y, Xu J, Han R. Nucleic Acids Res. 2016 Oct 30. pii: gkw1048. PubMed |
Rewiring human cellular input-output using modular extracellular sensors.
Schwarz KA, Daringer NM, Dolberg TB, Leonard JN.
Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
PubMed
Associated Plasmids |
Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening.
Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F.
Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23.
PubMed
Associated Plasmids |
| The Effects of Voluntary Physical Exercise-Activated Neurotrophic Signaling in Rat Hippocampus on mRNA Levels of Downstream Signaling Molecules. Solvsten CAE, Daugaard TF, Luo Y, de Paoli F, Christensen JH, Nielsen AL. J Mol Neurosci. 2017 Jun;62(2):142-153. doi: 10.1007/s12031-017-0918-9. Epub 2017 Apr 24. PubMed |
| A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma. Tammela T, Sanchez-Rivera FJ, Cetinbas NM, Wu K, Joshi NS, Helenius K, Park Y, Azimi R, Kerper NR, Wesselhoeft RA, Gu X, Schmidt L, Cornwall-Brady M, Yilmaz OH, Xue W, Katajisto P, Bhutkar A, Jacks T. Nature. 2017 May 18;545(7654):355-359. doi: 10.1038/nature22334. Epub 2017 May 10. PubMed |
| RNA-Guided Activation of Pluripotency Genes in Human Fibroblasts. Xiong K, Zhou Y, Blichfeld KA, Hyttel P, Bolund L, Freude KK, Luo Y. Cell Reprogram. 2017 Jun;19(3):189-198. doi: 10.1089/cell.2017.0006. PubMed |
| CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations. Kuscu C, Parlak M, Tufan T, Yang J, Szlachta K, Wei X, Mammadov R, Adli M. Nat Methods. 2017 Jul;14(7):710-712. doi: 10.1038/nmeth.4327. Epub 2017 Jun 5. PubMed |
| Quantitative proteomics identify Tenascin-C as a promoter of lung cancer progression and contributor to a signature prognostic of patient survival. Gocheva V, Naba A, Bhutkar A, Guardia T, Miller KM, Li CM, Dayton TL, Sanchez-Rivera FJ, Kim-Kiselak C, Jailkhani N, Winslow MM, Del Rosario A, Hynes RO, Jacks T. Proc Natl Acad Sci U S A. 2017 Jul 11;114(28):E5625-E5634. doi: 10.1073/pnas.1707054114. Epub 2017 Jun 26. PubMed |
| EINCR1 is an EGF inducible lincRNA overexpressed in lung adenocarcinomas. Nowicki-Osuch K, Li Y, Challinor M, Gerrard DT, Hanley NA, Sharrocks AD. PLoS One. 2017 Jul 21;12(7):e0181902. doi: 10.1371/journal.pone.0181902. eCollection 2017. PubMed |
| Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood. Joung J, Engreitz JM, Konermann S, Abudayyeh OO, Verdine VK, Aguet F, Gootenberg JS, Sanjana NE, Wright JB, Fulco CP, Tseng YY, Yoon CH, Boehm JS, Lander ES, Zhang F. Nature. 2017 Aug 11. doi: 10.1038/nature23451. PubMed |
| Specific Expression of Interferon-gamma Induced by Synergistic Activation Mediator-Derived Systems Activates Innate Immunity and Inhibits Tumorigenesis. Liu S, Yu X, Wang Q, Liu Z, Xiao Q, Hou P, Hu Y, Hou W, Yang Z, Guo D, Chen S. J Microbiol Biotechnol. 2017 Oct 28;27(10):1855-1866. doi: 10.4014/jmb.1705.05081. PubMed |
One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes.
Li S, Zhang A, Xue H, Li D, Liu Y.
Mol Ther Nucleic Acids. 2017 Sep 15;8:64-76. doi: 10.1016/j.omtn.2017.06.007. Epub 2017 Jun 15.
PubMed
Associated Plasmids |
| Ebola virus requires phosphatidylinositol (3,5) bisphosphate production for efficient viral entry. Qiu S, Leung A, Bo Y, Kozak RA, Anand SP, Warkentin C, Salambanga FDR, Cui J, Kobinger G, Kobasa D, Cote M. Virology. 2018 Jan 1;513:17-28. doi: 10.1016/j.virol.2017.09.028. Epub 2017 Oct 12. PubMed |
| Multiscale 3D Genome Rewiring during Mouse Neural Development. Bonev B, Mendelson Cohen N, Szabo Q, Fritsch L, Papadopoulos GL, Lubling Y, Xu X, Lv X, Hugnot JP, Tanay A, Cavalli G. Cell. 2017 Oct 19;171(3):557-572.e24. doi: 10.1016/j.cell.2017.09.043. PubMed |
| Conserved Gene Microsynteny Unveils Functional Interaction Between Protein Disulfide Isomerase and Rho Guanine-Dissociation Inhibitor Families. Moretti AIS, Pavanelli JC, Nolasco P, Leisegang MS, Tanaka LY, Fernandes CG, Wosniak J Jr, Kajihara D, Dias MH, Fernandes DC, Jo H, Tran NV, Ebersberger I, Brandes RP, Bonatto D, Laurindo FRM. Sci Rep. 2017 Dec 8;7(1):17262. doi: 10.1038/s41598-017-16947-5. PubMed |
| Rapid chromatin repression by Aire provides precise control of immune tolerance. Koh AS, Miller EL, Buenrostro JD, Moskowitz DM, Wang J, Greenleaf WJ, Chang HY, Crabtree GR. Nat Immunol. 2018 Feb;19(2):162-172. doi: 10.1038/s41590-017-0032-8. Epub 2018 Jan 15. PubMed |
| Polycomb protein RING1A limits hematopoietic differentiation in myelodysplastic syndromes. Palau A, Garz AK, Diesch J, Zwick A, Malinverni R, Valero V, Lappin K, Casquero R, Lennartsson A, Zuber J, Navarro T, Mills KI, Gotze KS, Buschbeck M. Oncotarget. 2017 Dec 1;8(70):115002-115017. doi: 10.18632/oncotarget.22839. eCollection 2017 Dec 29. PubMed |
| RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells. Guallar D, Bi X, Pardavila JA, Huang X, Saenz C, Shi X, Zhou H, Faiola F, Ding J, Haruehanroengra P, Yang F, Li D, Sanchez-Priego C, Saunders A, Pan F, Valdes VJ, Kelley K, Blanco MG, Chen L, Wang H, Sheng J, Xu M, Fidalgo M, Shen X, Wang J. Nat Genet. 2018 Mar;50(3):443-451. doi: 10.1038/s41588-018-0060-9. Epub 2018 Feb 26. PubMed |
| TCF21/POD-1, a Transcritional Regulator of SF-1/NR5A1, as a Potential Prognosis Marker in Adult and Pediatric Adrenocortical Tumors. Passaia BDS, Dias MH, Kremer JL, Antonini SRR, de Almeida MQ, Fragoso MCBV, Lotfi CFP. Front Endocrinol (Lausanne). 2018 Feb 22;9:38. doi: 10.3389/fendo.2018.00038. eCollection 2018. PubMed |
| Promoter of lncRNA Gene PVT1 Is a Tumor-Suppressor DNA Boundary Element. Cho SW, Xu J, Sun R, Mumbach MR, Carter AC, Chen YG, Yost KE, Kim J, He J, Nevins SA, Chin SF, Caldas C, Liu SJ, Horlbeck MA, Lim DA, Weissman JS, Curtis C, Chang HY. Cell. 2018 May 31;173(6):1398-1412.e22. doi: 10.1016/j.cell.2018.03.068. Epub 2018 May 3. PubMed |
| Epigenetic regulation of the circadian gene Per1 contributes to age-related changes in hippocampal memory. Kwapis JL, Alaghband Y, Kramar EA, Lopez AJ, Vogel Ciernia A, White AO, Shu G, Rhee D, Michael CM, Montellier E, Liu Y, Magnan CN, Chen S, Sassone-Corsi P, Baldi P, Matheos DP, Wood MA. Nat Commun. 2018 Aug 20;9(1):3323. doi: 10.1038/s41467-018-05868-0. PubMed |
| Comprehensive off-target analysis of dCas9-SAM-mediated HIV reactivation via long noncoding RNA and mRNA profiling. Zhang Y, Arango G, Li F, Xiao X, Putatunda R, Yu J, Yang XF, Wang H, Watson LT, Zhang L, Hu W. BMC Med Genomics. 2018 Sep 10;11(1):78. doi: 10.1186/s12920-018-0394-2. PubMed |
| Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Fauster A, Rebsamen M, Willmann KL, Cesar-Razquin A, Girardi E, Bigenzahn JW, Schischlik F, Scorzoni S, Bruckner M, Konecka J, Hormann K, Heinz LX, Boztug K, Superti-Furga G. Cell Death Differ. 2018 Sep 20. pii: 10.1038/s41418-018-0192-6. doi: 10.1038/s41418-018-0192-6. PubMed |
Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Liu LD, Huang M, Dai P, Liu T, Fan S, Cheng X, Zhao Y, Yeap LS, Meng FL.
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.
PubMed
Associated Plasmids |
| Genomic sequencing and editing revealed the GRM8 signaling pathway as potential therapeutic targets of squamous cell lung cancer. Zhang P, Kang B, Xie G, Li S, Gu Y, Shen Y, Zhao X, Ma Y, Li F, Si J, Wang J, Chen J, Yang H, Xu X, Yang Y. Cancer Lett. 2019 Feb 1;442:53-67. doi: 10.1016/j.canlet.2018.10.035. Epub 2018 Oct 27. PubMed |
| Pharmacological reactivation of MYC-dependent apoptosis induces susceptibility to anti-PD-1 immunotherapy. Haikala HM, Anttila JM, Marques E, Raatikainen T, Ilander M, Hakanen H, Ala-Hongisto H, Savelius M, Balboa D, Von Eyss B, Eskelinen V, Munne P, Nieminen AI, Otonkoski T, Schuler J, Laajala TD, Aittokallio T, Sihto H, Mattson J, Heikkila P, Leidenius M, Joensuu H, Mustjoki S, Kovanen P, Eilers M, Leverson JD, Klefstrom J. Nat Commun. 2019 Feb 6;10(1):620. doi: 10.1038/s41467-019-08541-2. PubMed |
Reduced mitochondrial fusion and Huntingtin levels contribute to impaired dendritic maturation and behavioral deficits in Fmr1-mutant mice.
Shen M, Wang F, Li M, Sah N, Stockton ME, Tidei JJ, Gao Y, Korabelnikov T, Kannan S, Vevea JD, Chapman ER, Bhattacharyya A, van Praag H, Zhao X.
Nat Neurosci. 2019 Mar;22(3):386-400. doi: 10.1038/s41593-019-0338-y. Epub 2019 Feb 11.
PubMed
Associated Plasmids |
| Construction and validation of the CRISPR/dCas9-EZH2 system for targeted H3K27Me3 modification. Chen X, Wei M, Liu X, Song S, Wang L, Yang X, Song Y. Biochem Biophys Res Commun. 2019 Apr 2;511(2):246-252. doi: 10.1016/j.bbrc.2019.02.011. Epub 2019 Feb 20. PubMed |
| CRISPR-mediated activation of endogenous BST-2/tetherin expression inhibits wild-type HIV-1 production. Zhang Y, Ozono S, Yao W, Tobiume M, Yamaoka S, Kishigami S, Fujita H, Tokunaga K. Sci Rep. 2019 Feb 28;9(1):3134. doi: 10.1038/s41598-019-40003-z. PubMed |
| Optimization of lentiviral transduction parameters and its application for CRISPR-based secretome modification of human endometrial mesenchymal stem cells. Deryabin P, Griukova A, Shatrova A, Petukhov A, Nikolsky N, Borodkina A. Cell Cycle. 2019 Mar - Apr;18(6-7):742-758. doi: 10.1080/15384101.2019.1593650. Epub 2019 Mar 28. PubMed |
| PI3K Inhibition Activates SGK1 via a Feedback Loop to Promote Chromatin-Based Regulation of ER-Dependent Gene Expression. Toska E, Castel P, Chhangawala S, Arruabarrena-Aristorena A, Chan C, Hristidis VC, Cocco E, Sallaku M, Xu G, Park J, Minuesa G, Shifman SG, Socci ND, Koche R, Leslie CS, Scaltriti M, Baselga J. Cell Rep. 2019 Apr 2;27(1):294-306.e5. doi: 10.1016/j.celrep.2019.02.111. PubMed |
| Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs. Pontis J, Planet E, Offner S, Turelli P, Duc J, Coudray A, Theunissen TW, Jaenisch R, Trono D. Cell Stem Cell. 2019 May 2;24(5):724-735.e5. doi: 10.1016/j.stem.2019.03.012. Epub 2019 Apr 18. PubMed |
Ezh2-dCas9 and KRAB-dCas9 enable engineering of epigenetic memory in a context-dependent manner.
O'Geen H, Bates SL, Carter SS, Nisson KA, Halmai J, Fink KD, Rhie SK, Farnham PJ, Segal DJ.
Epigenetics Chromatin. 2019 May 3;12(1):26. doi: 10.1186/s13072-019-0275-8.
PubMed
Associated Plasmids |
| FOXA1 mutations alter pioneering activity, differentiation and prostate cancer phenotypes. Adams EJ, Karthaus WR, Hoover E, Liu D, Gruet A, Zhang Z, Cho H, DiLoreto R, Chhangawala S, Liu Y, Watson PA, Davicioni E, Sboner A, Barbieri CE, Bose R, Leslie CS, Sawyers CL. Nature. 2019 Jul;571(7765):408-412. doi: 10.1038/s41586-019-1318-9. Epub 2019 Jun 26. PubMed |
| Notum produced by Paneth cells attenuates regeneration of aged intestinal epithelium. Pentinmikko N, Iqbal S, Mana M, Andersson S, Cognetta AB 3rd, Suciu RM, Roper J, Luopajarvi K, Markelin E, Gopalakrishnan S, Smolander OP, Naranjo S, Saarinen T, Juuti A, Pietilainen K, Auvinen P, Ristimaki A, Gupta N, Tammela T, Jacks T, Sabatini DM, Cravatt BF, Yilmaz OH, Katajisto P. Nature. 2019 Jul;571(7765):398-402. doi: 10.1038/s41586-019-1383-0. Epub 2019 Jul 10. PubMed |
| BEAT: A Python Program to Quantify Base Editing from Sanger Sequencing. Xu L, Liu Y, Han R. CRISPR J. 2019 Aug;2:223-229. doi: 10.1089/crispr.2019.0017. Epub 2019 Jul 18. PubMed |
| Functional genomics identifies predictive markers and clinically actionable resistance mechanisms to CDK4/6 inhibition in bladder cancer. Tong Z, Sathe A, Ebner B, Qi P, Veltkamp C, Gschwend JE, Holm PS, Nawroth R. J Exp Clin Cancer Res. 2019 Jul 22;38(1):322. doi: 10.1186/s13046-019-1322-9. PubMed |
| MUC16 in non-small cell lung cancer patients affected by familial lung cancer and indoor air pollution: clinical characteristics and cell behaviors. Chen Y, Huang Y, Kanwal M, Li G, Yang J, Niu H, Li Z, Ding X. Transl Lung Cancer Res. 2019 Aug;8(4):476-488. doi: 10.21037/tlcr.2019.07.10. PubMed |
| H3K18ac Primes Mesendodermal Differentiation upon Nodal Signaling. Luo M, Bai J, Liu B, Yan P, Zuo F, Sun H, Sun Y, Xu X, Song Z, Yang Y, Massague J, Lan X, Lu Z, Chen YG, Deng H, Xie W, Xi Q. Stem Cell Reports. 2019 Oct 8;13(4):642-656. doi: 10.1016/j.stemcr.2019.08.016. Epub 2019 Sep 26. PubMed |
| Transcriptional landscape and clinical utility of enhancer RNAs for eRNA-targeted therapy in cancer. Zhang Z, Lee JH, Ruan H, Ye Y, Krakowiak J, Hu Q, Xiang Y, Gong J, Zhou B, Wang L, Lin C, Diao L, Mills GB, Li W, Han L. Nat Commun. 2019 Oct 8;10(1):4562. doi: 10.1038/s41467-019-12543-5. PubMed |
| lncRNA RMST Enhances DNMT3 Expression through Interaction with HuR. Peng WX, Koirala P, Zhang W, Ni C, Wang Z, Yang L, Mo YY. Mol Ther. 2020 Jan 8;28(1):9-18. doi: 10.1016/j.ymthe.2019.09.024. Epub 2019 Oct 4. PubMed |
| Molecular basis of senescence transmitting in the population of human endometrial stromal cells. Griukova A, Deryabin P, Shatrova A, Burova E, Severino V, Farina A, Nikolsky N, Borodkina A. Aging (Albany NY). 2019 Nov 5;11(21):9912-9931. doi: 10.18632/aging.102441. Epub 2019 Nov 5. PubMed |
| Distinct IL-1alpha-responsive enhancers promote acute and coordinated changes in chromatin topology in a hierarchical manner. Weiterer SS, Meier-Soelch J, Georgomanolis T, Mizi A, Beyerlein A, Weiser H, Brant L, Mayr-Buro C, Jurida L, Beuerlein K, Muller H, Weber A, Tenekeci U, Dittrich-Breiholz O, Bartkuhn M, Nist A, Stiewe T, van IJcken WF, Riedlinger T, Schmitz ML, Papantonis A, Kracht M. EMBO J. 2020 Jan 2;39(1):e101533. doi: 10.15252/embj.2019101533. Epub 2019 Nov 7. PubMed |
| Dose-dependent activation of gene expression is achieved using CRISPR and small molecules that recruit endogenous chromatin machinery. Chiarella AM, Butler KV, Gryder BE, Lu D, Wang TA, Yu X, Pomella S, Khan J, Jin J, Hathaway NA. Nat Biotechnol. 2020 Jan;38(1):50-55. doi: 10.1038/s41587-019-0296-7. Epub 2019 Nov 11. PubMed |
| The targetable kinase PIM1 drives ALK inhibitor resistance in high-risk neuroblastoma independent of MYCN status. Trigg RM, Lee LC, Prokoph N, Jahangiri L, Reynolds CP, Amos Burke GA, Probst NA, Han M, Matthews JD, Lim HK, Manners E, Martinez S, Pastor J, Blanco-Aparicio C, Merkel O, de Los Fayos Alonso IG, Kodajova P, Tangermann S, Hogler S, Luo J, Kenner L, Turner SD. Nat Commun. 2019 Nov 28;10(1):5428. doi: 10.1038/s41467-019-13315-x. PubMed |
| Gene activation by dCas9-CBP and the SAM system differ in target preference. Sajwan S, Mannervik M. Sci Rep. 2019 Dec 2;9(1):18104. doi: 10.1038/s41598-019-54179-x. PubMed |
| Induction of an Alternative mRNA 5' Leader Enhances Translation of the Ciliopathy Gene Inpp5e and Resistance to Oncolytic Virus Infection. Hoang HD, Graber TE, Jia JJ, Vaidya N, Gilchrist VH, Xiang X, Li W, Cowan KN, Gkogkas CG, Jaramillo M, Jafarnejad SM, Alain T. Cell Rep. 2019 Dec 17;29(12):4010-4023.e5. doi: 10.1016/j.celrep.2019.11.072. PubMed |
| CRISPR-Cas9-induced IGF1 gene activation as a tool for enhancing muscle differentiation via multiple isoform expression. Roberston MJ, Raghunathan S, Potaman VN, Zhang F, Stewart MD, McConnell BK, Schwartz RJ. FASEB J. 2020 Jan;34(1):555-570. doi: 10.1096/fj.201901107RR. Epub 2019 Nov 25. PubMed |
Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing.
Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson KE, Ni M, Xu J.
Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5.
PubMed
Associated Plasmids |
| Cancer Cell-Derived Matrisome Proteins Promote Metastasis in Pancreatic Ductal Adenocarcinoma. Tian C, Ohlund D, Rickelt S, Lidstrom T, Huang Y, Hao L, Zhao RT, Franklin O, Bhatia SN, Tuveson DA, Hynes RO. Cancer Res. 2020 Apr 1;80(7):1461-1474. doi: 10.1158/0008-5472.CAN-19-2578. Epub 2020 Feb 6. PubMed |
| DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State. Yang F, Huang X, Zang R, Chen J, Fidalgo M, Sanchez-Priego C, Yang J, Caichen A, Ma F, Macfarlan T, Wang H, Gao S, Zhou H, Wang J. Cell Stem Cell. 2020 Feb 6;26(2):234-250.e7. doi: 10.1016/j.stem.2020.01.004. PubMed |
| Chromosome 19 microRNA cluster enhances cell reprogramming by inhibiting epithelial-to-mesenchymal transition. Mong EF, Yang Y, Akat KM, Canfield J, VanWye J, Lockhart J, Tsibris JCM, Schatz F, Lockwood CJ, Tuschl T, Kayisli UA, Totary-Jain H. Sci Rep. 2020 Feb 20;10(1):3029. doi: 10.1038/s41598-020-59812-8. PubMed |
| Lineage reprogramming of fibroblasts into induced cardiac progenitor cells by CRISPR/Cas9-based transcriptional activators. Wang J, Jiang X, Zhao L, Zuo S, Chen X, Zhang L, Lin Z, Zhao X, Qin Y, Zhou X, Yu XY. Acta Pharm Sin B. 2020 Feb;10(2):313-326. doi: 10.1016/j.apsb.2019.09.003. Epub 2019 Sep 17. PubMed |
| Noncoding Variants Connect Enhancer Dysregulation with Nuclear Receptor Signaling in Hematopoietic Malignancies. Li K, Zhang Y, Liu X, Liu Y, Gu Z, Cao H, Dickerson KE, Chen M, Chen W, Shao Z, Ni M, Xu J. Cancer Discov. 2020 May;10(5):724-745. doi: 10.1158/2159-8290.CD-19-1128. Epub 2020 Mar 18. PubMed |
| LRP6-CRISPR prevents activation of hepatic stellate cells and liver fibrogenesis in rats. Yu L, Wang L, Yi H, Wu X. Am J Transl Res. 2020 Feb 15;12(2):397-408. eCollection 2020. PubMed |
| BEON: A Functional Fluorescence Reporter for Quantification and Enrichment of Adenine Base-Editing Activity. Wang P, Xu L, Gao Y, Han R. Mol Ther. 2020 Jul 8;28(7):1696-1705. doi: 10.1016/j.ymthe.2020.04.009. Epub 2020 Apr 16. PubMed |
| Filoviruses Use the HOPS Complex and UVRAG To Traffic to Niemann-Pick C1 Compartments during Viral Entry. Bo Y, Qiu S, Mulloy RP, Cote M. J Virol. 2020 Jul 30;94(16). pii: JVI.01002-20. doi: 10.1128/JVI.01002-20. Print 2020 Jul 30. PubMed |
| A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by Treg cells. Nasrallah R, Imianowski CJ, Bossini-Castillo L, Grant FM, Dogan M, Placek L, Kozhaya L, Kuo P, Sadiyah F, Whiteside SK, Mumbach MR, Glinos D, Vardaka P, Whyte CE, Lozano T, Fujita T, Fujii H, Liston A, Andrews S, Cozzani A, Yang J, Mitra S, Lugli E, Chang HY, Unutmaz D, Trynka G, Roychoudhuri R. Nature. 2020 Jul;583(7816):447-452. doi: 10.1038/s41586-020-2296-7. Epub 2020 May 13. PubMed |
| Enforced Activation of Enhancer RNAs In Situ through the dCas9 Synergistic Activation Mediator System. Liao Z, Lee JH, Krakowiak J, Wang R, Li W. J Vis Exp. 2020 Jun 5;(160). doi: 10.3791/61460. PubMed |
| Nonsense-mediated decay factor SMG7 sensitizes cells to TNFalpha-induced apoptosis via CYLD tumor suppressor and the noncoding oncogene Pvt1. Yang L, Kraft VAN, Pfeiffer S, Merl-Pham J, Bao X, An Y, Hauck SM, Schick JA. Mol Oncol. 2020 Jun 30. doi: 10.1002/1878-0261.12754. PubMed |
| SLE non-coding genetic risk variant determines the epigenetic dysfunction of an immune cell specific enhancer that controls disease-critical microRNA expression. Hou G, Harley ITW, Lu X, Zhou T, Xu N, Yao C, Qin Y, Ouyang Y, Ma J, Zhu X, Yu X, Xu H, Dai D, Ding H, Yin Z, Ye Z, Deng J, Zhou M, Tang Y, Namjou B, Guo Y, Weirauch MT, Kottyan LC, Harley JB, Shen N. Nat Commun. 2021 Jan 8;12(1):135. doi: 10.1038/s41467-020-20460-1. PubMed |
| Advanced single-cell pooled CRISPR screening identifies C19orf53 required for cell proliferation based on mTORC1 regulators. Tang Y, Liao S, Liu G, Xiong X, Liu H, Li F, Tan Z, Kong X, Yin Y, Tan B. Cell Biol Toxicol. 2021 Feb 13. pii: 10.1007/s10565-021-09586-0. doi: 10.1007/s10565-021-09586-0. PubMed |
| HIF1alpha epigenetically repressed macrophages via CRISPR/Cas9-EZH2 system for enhanced cancer immunotherapy. Dong Y, Zhang S, Gao X, Yin D, Wang T, Li Z, Wan Z, Wei M, Luo Y, Yang G, Liu L. Bioact Mater. 2021 Feb 20;6(9):2870-2880. doi: 10.1016/j.bioactmat.2021.02.008. eCollection 2021 Sep. PubMed |
| The NFIB-ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells. Zilli F, Marques Ramos P, Auf der Maur P, Jehanno C, Sethi A, Coissieux MM, Eichlisberger T, Sauteur L, Rouchon A, Bonapace L, Pinto Couto J, Rad R, Jensen MR, Banfi A, Stadler MB, Bentires-Alj M. EMBO Mol Med. 2021 Apr 9;13(4):e13162. doi: 10.15252/emmm.202013162. Epub 2021 Mar 10. PubMed |
Proteasomal degradation of the tumour suppressor FBW7 requires branched ubiquitylation by TRIP12.
Khan OM, Almagro J, Nelson JK, Horswell S, Encheva V, Keyan KS, Clurman BE, Snijders AP, Behrens A.
Nat Commun. 2021 Apr 6;12(1):2043. doi: 10.1038/s41467-021-22319-5.
PubMed
Associated Plasmids |
| Suppression of pancreatic ductal adenocarcinoma growth and metastasis by fibrillar collagens produced selectively by tumor cells. Tian C, Huang Y, Clauser KR, Rickelt S, Lau AN, Carr SA, Vander Heiden MG, Hynes RO. Nat Commun. 2021 Apr 20;12(1):2328. doi: 10.1038/s41467-021-22490-9. PubMed |
| RNA m(6)A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability. Xiong F, Wang R, Lee JH, Li S, Chen SF, Liao Z, Hasani LA, Nguyen PT, Zhu X, Krakowiak J, Lee DF, Han L, Tsai KL, Liu Y, Li W. Cell Res. 2021 Aug;31(8):861-885. doi: 10.1038/s41422-021-00515-8. Epub 2021 Jun 9. PubMed |
| SARS-CoV-2 Restructures the Host Chromatin Architecture. Wang R, Lee JH, Xiong F, Kim J, Hasani LA, Yuan X, Shivshankar P, Krakowiak J, Qi C, Wang Y, Eltzschig HK, Li W. bioRxiv. 2021 Jul 21. doi: 10.1101/2021.07.20.453146. PubMed |
| MS4A15 drives ferroptosis resistance through calcium-restricted lipid remodeling. Xin S, Mueller C, Pfeiffer S, Kraft VAN, Merl-Pham J, Bao X, Feederle R, Jin X, Hauck SM, Schmitt-Kopplin P, Schick JA. Cell Death Differ. 2022 Mar;29(3):670-686. doi: 10.1038/s41418-021-00883-z. Epub 2021 Oct 18. PubMed |
| Lnc-DC promotes estrogen independent growth and tamoxifen resistance in breast cancer. Peng WX, Koirala P, Zhou H, Jiang J, Zhang Z, Yang L, Mo YY. Cell Death Dis. 2021 Oct 25;12(11):1000. doi: 10.1038/s41419-021-04288-1. PubMed |
Rapid genome editing by CRISPR-Cas9-POLD3 fusion.
Reint G, Li Z, Labun K, Keskitalo S, Soppa I, Mamia K, Tolo E, Szymanska M, Meza-Zepeda LA, Lorenz S, Cieslar-Pobuda A, Hu X, Bordin DL, Staerk J, Valen E, Schmierer B, Varjosalo M, Taipale J, Haapaniemi E.
Elife. 2021 Dec 13;10. pii: 75415. doi: 10.7554/eLife.75415.
PubMed
Associated Plasmids |
| Upregulation of CD14 in mesenchymal stromal cells accelerates lipopolysaccharide-induced response and enhances antibacterial properties. Hirakawa MP, Tjahjono N, Light YK, Celebi AN, Celebi NN, Chintalapudi P, Butler KS, Branda SS, Krishnakumar R. iScience. 2022 Jan 12;25(2):103759. doi: 10.1016/j.isci.2022.103759. eCollection 2022 Feb 18. PubMed |
| Bioorthogonal Chemical Epigenetic Modifiers Enable Dose-Dependent CRISPR Targeted Gene Activation in Mammalian Cells. Lu D, Foley CA, Birla SV, Hepperla AJ, Simon JM, James LI, Hathaway NA. ACS Synth Biol. 2022 Apr 15;11(4):1397-1407. doi: 10.1021/acssynbio.1c00606. Epub 2022 Mar 18. PubMed |
| ABCA1 Expression Is Upregulated in an EMT in Breast Cancer Cell Lines via MYC-Mediated De-Repression of Its Proximal Ebox Element. Prijic S, Chang JT. Biomedicines. 2022 Mar 2;10(3). pii: biomedicines10030581. doi: 10.3390/biomedicines10030581. PubMed |
| CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Joung J, Kirchgatterer PC, Singh A, Cho JH, Nety SP, Larson RC, Macrae RK, Deasy R, Tseng YY, Maus MV, Zhang F. Nat Commun. 2022 Mar 25;13(1):1606. doi: 10.1038/s41467-022-29205-8. PubMed |
| Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery. Zhou T, Zhu X, Ye Z, Wang YF, Yao C, Xu N, Zhou M, Ma J, Qin Y, Shen Y, Tang Y, Yin Z, Xu H, Zhang Y, Zang X, Ding H, Yang W, Guo Y, Harley JB, Namjou B, Kaufman KM, Kottyan LC, Weirauch MT, Hou G, Shen N. Nat Commun. 2022 Apr 6;13(1):1855. doi: 10.1038/s41467-022-29514-y. PubMed |
The nuclear receptor THRB facilitates differentiation of human PSCs into more mature hepatocytes.
Ma H, de Zwaan E, Guo YE, Cejas P, Thiru P, van de Bunt M, Jeppesen JF, Syamala S, Dall'Agnese A, Abraham BJ, Fu D, Garrett-Engele C, Lee TI, Long HW, Griffith LG, Young RA, Jaenisch R.
Cell Stem Cell. 2022 Apr 13. pii: S1934-5909(22)00150-3. doi: 10.1016/j.stem.2022.03.015.
PubMed
Associated Plasmids |
| 3D Chromatin Organization Involving MEIS1 Factor in the cis-Regulatory Landscape of GJB2. Le Nabec A, Blotas C, Briset A, Collobert M, Ferec C, Moisan S. Int J Mol Sci. 2022 Jun 23;23(13). pii: ijms23136964. doi: 10.3390/ijms23136964. PubMed |
| A Nodal enhanced micropeptide NEMEP regulates glucose uptake during mesendoderm differentiation of embryonic stem cells. Fu H, Wang T, Kong X, Yan K, Yang Y, Cao J, Yuan Y, Wang N, Kee K, Lu ZJ, Xi Q. Nat Commun. 2022 Jul 9;13(1):3984. doi: 10.1038/s41467-022-31762-x. PubMed |
| Constitutive heterochromatin propagation contributes to the X chromosome inactivation. Shevchenko AI, Rifel NA, Zakian SM, Zakharova IS. Chromosome Res. 2022 Dec;30(4):289-307. doi: 10.1007/s10577-022-09706-4. Epub 2022 Aug 3. PubMed |
Gain-of-function genetic screens in human cells identify SLC transporters overcoming environmental nutrient restrictions.
Rebsamen M, Girardi E, Sedlyarov V, Scorzoni S, Papakostas K, Vollert M, Konecka J, Guertl B, Klavins K, Wiedmer T, Superti-Furga G.
Life Sci Alliance. 2022 Sep 16;5(11):e202201404. doi: 10.26508/lsa.202201404. Print 2022 Nov.
PubMed
Associated Plasmids |
The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A.
Beucher A, Miguel-Escalada I, Balboa D, De Vas MG, Maestro MA, Garcia-Hurtado J, Bernal A, Gonzalez-Franco R, Vargiu P, Heyn H, Ravassard P, Ortega S, Ferrer J.
Nat Cell Biol. 2022 Oct 6. pii: 10.1038/s41556-022-00996-8. doi: 10.1038/s41556-022-00996-8.
PubMed
Associated Plasmids |
| Integrative Functional Genomics Identifies Systemic Lupus Erythematosus Causal Genetic Variant in the IRF5 Risk Locus. Hou G, Zhou T, Xu N, Yin Z, Zhu X, Zhang Y, Cui Y, Ma J, Tang Y, Cheng Z, Shen Y, Chen Y, Zou LH, Wang YF, Yin Z, Guo Y, Ding H, Ye Z, Shen N. Arthritis Rheumatol. 2023 Apr;75(4):574-585. doi: 10.1002/art.42390. Epub 2023 Feb 8. PubMed |
| KCNQ1OT1 promotes genome-wide transposon repression by guiding RNA-DNA triplexes and HP1 binding. Zhang X, Jiang Q, Li J, Zhang S, Cao Y, Xia X, Cai D, Tan J, Chen J, Han JJ. Nat Cell Biol. 2022 Nov;24(11):1617-1629. doi: 10.1038/s41556-022-01008-5. Epub 2022 Oct 20. PubMed |
| Acute depletion of human core nucleoporin reveals direct roles in transcription control but dispensability for 3D genome organization. Zhu X, Qi C, Wang R, Lee JH, Shao J, Bei L, Xiong F, Nguyen PT, Li G, Krakowiak J, Koh SP, Simon LM, Han L, Moore TI, Li W. Cell Rep. 2022 Nov 1;41(5):111576. doi: 10.1016/j.celrep.2022.111576. PubMed |
| Identification of NRAS Downstream Genes with CRISPR Activation Screening. Tatsumi A, Hirakochi H, Inoue S, Tanaka Y, Furuno H, Ikeda M, Ishibashi S, Taguchi T, Yamamoto K, Onishi I, Sachs Z, Largaespada DA, Kitagawa M, Kurata M. Biology (Basel). 2022 Oct 23;11(11):1551. doi: 10.3390/biology11111551. PubMed |
| Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8. Wang N, He J, Feng X, Liao S, Zhao Y, Tang F, Kee K. Elife. 2023 Jan 5;12:e78421. doi: 10.7554/eLife.78421. PubMed |
| Functional Validation of the Putative Oncogenic Activity of PLAU. Sarno F, Goubert D, Logie E, Rutten MGS, Koncz M, Deben C, Niemarkt AE, Altucci L, Verschure PJ, Kiss A, Berghe WV, Rots MG. Biomedicines. 2022 Dec 30;11(1):102. doi: 10.3390/biomedicines11010102. PubMed |
| Multimodal CRISPR perturbations of GWAS loci associated with coronary artery disease in vascular endothelial cells. Wunnemann F, Fotsing Tadjo T, Beaudoin M, Lalonde S, Lo KS, Kleinstiver BP, Lettre G. PLoS Genet. 2023 Mar 16;19(3):e1010680. doi: 10.1371/journal.pgen.1010680. eCollection 2023 Mar. PubMed |
| SARS-CoV-2 restructures host chromatin architecture. Wang R, Lee JH, Kim J, Xiong F, Hasani LA, Shi Y, Simpson EN, Zhu X, Chen YT, Shivshankar P, Krakowiak J, Wang Y, Gilbert DM, Yuan X, Eltzschig HK, Li W. Nat Microbiol. 2023 Apr;8(4):679-694. doi: 10.1038/s41564-023-01344-8. Epub 2023 Mar 23. PubMed |
| Functional mapping of microRNA promoters with dCas9 fused to transcriptional regulators. Kumar P, Courtes M, Lemmers C, Le Digarcher A, Coku I, Monteil A, Hong C, Varrault A, Liu R, Wang L, Bouschet T. Front Genet. 2023 May 5;14:1147222. doi: 10.3389/fgene.2023.1147222. eCollection 2023. PubMed |
| Nicotinamide N-methyltransferase sustains a core epigenetic program that promotes metastatic colonization in breast cancer. Couto JP, Vulin M, Jehanno C, Coissieux MM, Hamelin B, Schmidt A, Ivanek R, Sethi A, Brautigam K, Frei AL, Hager C, Manivannan M, Gomez-Miragaya J, Obradovic MM, Varga Z, Koelzer VH, Mertz KD, Bentires-Alj M. EMBO J. 2023 Jul 3;42(13):e112559. doi: 10.15252/embj.2022112559. Epub 2023 Jun 1. PubMed |
| Small Extracellular Vesicle-Derived vWF Induces a Positive Feedback Loop between Tumor and Endothelial Cells to Promote Angiogenesis and Metastasis in Hepatocellular Carcinoma. Wong SWK, Tey SK, Mao X, Fung HL, Xiao ZJ, Wong DKH, Mak LY, Yuen MF, Ng IO, Yun JP, Gao Y, Yam JWP. Adv Sci (Weinh). 2023 Sep;10(26):e2302677. doi: 10.1002/advs.202302677. Epub 2023 Jun 30. PubMed |
| Temporal analyses reveal a pivotal role for sense and antisense enhancer RNAs in coordinate immunoglobulin lambda locus activation. Gao Z, Smith AL, Scott JNF, Bevington SL, Boyes J. Nucleic Acids Res. 2023 Oct 27;51(19):10344-10363. doi: 10.1093/nar/gkad741. PubMed |
| Targeted epigenetic silencing of UCHL1 expression suppresses collagen-1 production in human lung epithelial cells. Wu DD, Lau ATY, Xu YM, Reinders-Luinge M, Koncz M, Kiss A, Timens W, Rots MG, Hylkema MN. Epigenetics. 2023 Dec;18(1):2175522. doi: 10.1080/15592294.2023.2175522. Epub 2023 Feb 22. PubMed |
| NHE7 upregulation potentiates the uptake of small extracellular vesicles by enhancing maturation of macropinosomes in hepatocellular carcinoma. Yao Y, Xu Y, Yu L, Xue TM, Xiao ZJ, Tin PC, Fung HL, Ma HT, Yun JP, Yam JWP. Cancer Commun (Lond). 2024 Feb;44(2):251-272. doi: 10.1002/cac2.12515. Epub 2023 Dec 28. PubMed |
If you have published an article using this material, please email us at [email protected] to have your article added to this page.