The nuclear receptor THRB facilitates differentiation of human PSCs into more mature hepatocytes.
Ma H, de Zwaan E, Guo YE, Cejas P, Thiru P, van de Bunt M, Jeppesen JF, Syamala S, Dall'Agnese A, Abraham BJ, Fu D, Garrett-Engele C, Lee TI, Long HW, Griffith LG, Young RA, Jaenisch R
Cell Stem Cell. 2022 Apr 13. pii: S1934-5909(22)00150-3. doi: 10.1016/j.stem.2022.03.015. PubMed Article
Ma H, de Zwaan E, Guo YE, Cejas P, Thiru P, van de Bunt M, Jeppesen JF, Syamala S, Dall'Agnese A, Abraham BJ, Fu D, Garrett-Engele C, Lee TI, Long HW, Griffith LG, Young RA, Jaenisch R
Cell Stem Cell. 2022 Apr 13. pii: S1934-5909(22)00150-3. doi: 10.1016/j.stem.2022.03.015. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176836 | DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct | Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus. |
| 176837 | Cre-p2a-tdTomato | Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression |
| 176838 | lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1 | To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter |
| 176839 | lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1 | To recruit SAM complex to human CYP3A4 proximal enhancer. |
| 176840 | 330-cherry-hCYP3A4-enhancer-L-sgRNA | Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression. |
| 176841 | 330-BFP-hCYP3A4-enhancer-R-sgRNA | Targeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression. |