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Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Liu LD, Huang M, Dai P, Liu T, Fan S, Cheng X, Zhao Y, Yeap LS, Meng FL
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
112127pLL1 sgRNA(MS2)-MS2-AIDmonoempty sgRNA cloning vector with MS2-AIDmono
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112128pLL2 sgRNA(MS2)-MS2-AID3cempty sgRNA cloning vector with MS2-AID3c
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112129pLL3 sgRNA(MS2)-MS2-AID3fempty sgRNA cloning vector with MS2-AID3f
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112130pLL4 sgRNA(MS2)-MS2-hA3aempty sgRNA cloning vector with MS2-humanA3a
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112131pLL5 sgRNA(MS2)-MS2-hA3a D131Aempty sgRNA cloning vector with MS2-humanA3a D131A
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112132pLL6 sgRNA(MS2)-MS2-hA3a D131Eempty sgRNA cloning vector with MS2-humanA3a D131E
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112133pLL7 lenti-dcas9-3xflag-blastlenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)
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112134pLL8 lenti-D10Acas9-3xflag-blastlenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)
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