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dCas9-DNMT3L
(Plasmid #126588)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 126588 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.3-TOPO
  • Backbone size w/o insert (bp) 5407
  • Total vector size (bp) 11080
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DNMT3L
  • Alt name
    DNA methyltransferase 3 like
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_175867.2
  • Entrez Gene
    DNMT3L
  • Tag / Fusion Protein
    • 3XFLag-NLS-dCas9-DNMT3L-NLS

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dCas9-DNMT3L was a gift from David Segal (Addgene plasmid # 126588 ; http://n2t.net/addgene:126588 ; RRID:Addgene_126588)
  • For your References section:

    Ezh2-dCas9 and KRAB-dCas9 enable engineering of epigenetic memory in a context-dependent manner. O'Geen H, Bates SL, Carter SS, Nisson KA, Halmai J, Fink KD, Rhie SK, Farnham PJ, Segal DJ. Epigenetics Chromatin. 2019 May 3;12(1):26. doi: 10.1186/s13072-019-0275-8. 10.1186/s13072-019-0275-8 PubMed 31053162