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Rewiring human cellular input-output using modular extracellular sensors.
Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
84500V1-MESA-45F-M-tTAMESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and tTA
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84501V1-MESA-45F-TevMESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev protease
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84502V2-MESA-35F-M-tTAMESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and tTA
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84503V2-MESA-35F-TevMESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev protease
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84504V1-MESA-45F-M-dCas9MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64
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84505V1-MESA-45F-Tev-MS2-p65-HSF1MESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev protease, MS2-P65-HSF1
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84506V2-MESA-35F-M-dCas9MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64
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84513V2-MESA-35F-Tev-MS2-p65-HSF1MESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev protease, MS2-P65-HSF1
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Recombinant Antibodies from Article