pLKO.1 - TRC control
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10879||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7032
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert namenon-hairpin 18bp
Insert Size (bp)18
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
This plasmid is recommended by The RNAi Consortium for use as a non-hairpin control http://www.broad.mit.edu/genome_bio/trc/ The 18bp between AgeI and EcoRI of this plasmid are CCGCAGGTATGCACGCGT. The link to the author's map shows the key features of this vector before the 18bp were cloned in.
Also, see Addgene's pLKO.1 protocol http://www.addgene.org/plko on how to use the pLKO.1 vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.1 - TRC control was a gift from David Root (Addgene plasmid # 10879 ; http://n2t.net/addgene:10879 ; RRID:Addgene_10879)
For your References section:A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, Piqani B, Eisenhaure TM, Luo B, Grenier JK, Carpenter AE, Foo SY, Stewart SA, Stockwell BR, Hacohen N, Hahn WC, Lander ES, Sabatini DM, Root DE. Cell. 2006 Mar 24. 124(6):1283-98. 10.1016/j.cell.2006.01.040 PubMed 16564017