PurposeIn vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26475||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4258
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)8646
- Cloning method Restriction Enzyme
- 5′ sequencing primer None (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDRGFP was a gift from Maria Jasin (Addgene plasmid # 26475 ; http://n2t.net/addgene:26475 ; RRID:Addgene_26475)
For your References section:XRCC3 promotes homology-directed repair of DNA damage in mammalian cells. Pierce AJ, Johnson RD, Thompson LH, Jasin M. Genes Dev. 1999 Oct 15. 13(20):2633-8. 10.1101/gad.13.20.2633 PubMed 10541549