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(Plasmid #44026)


Item Catalog # Description Quantity Price (USD)
Plasmid 44026 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    p59 pim targeting vector
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    EJ5GFP egfp-based chromosomal break reporter
  • Species
  • Mutation
    pCAGGS promoter and eGFP separated by pgkPURO cassette flanked by two I-SceI sites
  • GenBank ID
  • Promoter pCAGGS

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ttcctacagctcctgggcaacg
  • 3′ sequencing primer ttttggcagagggaaaaaga
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reporter for NHEJ. I-SceI-based chromosomal break reporter for end joining. End joining between two distal tandem I-SceI recognition sites restores an eGFP expression cassette, causing deletion of the intervening pgkPURO cassette. Linearize with XhoI for integration into any mammalian cell type. Also contains a promoter-less HYG gene and targeting arms to select for targeted integration to the mouse pim1 locus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pimEJ5GFP was a gift from Jeremy Stark (Addgene plasmid # 44026 ; ; RRID:Addgene_44026)
  • For your References section:

    Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair. Bennardo N, Cheng A, Huang N, Stark JM. PLoS Genet. 2008 Jun 27;4(6):e1000110. doi: 10.1371/journal.pgen.1000110. 10.1371/journal.pgen.1000110 PubMed 18584027