We narrowed to 16,198 results for: grna

1. shRNA-Ratio-PosCon

   Plasmid

   #183553

   Purpose

   Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio positive control (Pbrm1), Figure S2)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Pbrm1 shRNA
   

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. shRNA-Ratio-NegCon

   Plasmid

   #183554

   Purpose

   Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Luciferase shRNA
   

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTAG4E

   Plasmid

   #176868

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_4E
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTAG5

   Plasmid

   #176869

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_5
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTAG6E

   Plasmid

   #176870

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_6E
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTAG2

   Plasmid

   #176864

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_2
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTAG3

   Plasmid

   #176866

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_3
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTAG4

   Plasmid

   #176867

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_4
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLeGO.sgGata1.4.RUNX1A.iG2

   Plasmid

   #181976

   Purpose

   gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

   Depositor

   Jan-Henning Klusmann

   Inserts

   GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
   RUNX1A (RUNX1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-sg-CMV152

    Plasmid

    #179916

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX459-sg-CMV51

    Plasmid

    #179915

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX459-sg-CMV162

    Plasmid

    #179917

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-sg-mGreenLantern332

    Plasmid

    #179914

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PX552-rScn2a-3xV5-Syn-miRFP670

    Plasmid

    #182566

    Purpose

    For rat Nav1.2 knockin with 3xV5 at the extracellular loop

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    rat Scn2a gRNA and 3xV5 donor DNA
    miRFP670
    

    Use

    AAV and CRISPR

    Promoter

    Syn and U6

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMCL8

    Plasmid

    #176184

    Purpose

    gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMCL8_28bp

    Plasmid

    #176185

    Purpose

    plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMCL9

    Plasmid

    #176186

    Purpose

    2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6-TREX1 g3 Cas9-T2A-mCherry

    Plasmid

    #164252

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPPC008.106

    Plasmid

    #171141

    Purpose

    Expression of J106 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    J106 scRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJJF144_SECGFP_sg2

    Plasmid

    #163865

    Purpose

    sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting GFP
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    R07E5.16 (U6)

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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