179,539 results
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Plasmid#59205PurposeMammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloningDepositorTypeEmpty backboneUseLentiviral; DamidTagsDam (DNA adenine methyltransferase) and V5ExpressionMammalianPromoterHeat Shock Minimal PromoterAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pRK-flag-GFP11
Plasmid#78590PurposeExpression flag-GFP 11 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertflag-GFP 11
TagsflagExpressionMammalianPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH-tdGFP
Plasmid#74322PurposepH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiaeDepositorInsertpH-tdGFP
UseYeast taggingTagsnoneExpressionYeastMutationN149Y, Q204HAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBluescriptSKII+ U6-sgRNA(F+E) ACTB
Plasmid#74705PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
UseCRISPRExpressionMammalianAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
FU- dio Teal-gephyrin - W
Plasmid#73918PurposeTo express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.DepositorInsertTeal-gephyrin
UseLentiviralTagsTealExpressionMammalianAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-DDX3X
Plasmid#70648PurposeExpresses WT DDX3X in mammalian cells under blasticidin selection.DepositorPublicationAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
R777-E285 Hs.ROCK2
Plasmid#70569PurposeGateway ORF clone of human ROCK2 [NM_004850.3] with stop codon (for native or N-terminal fusions)DepositorInsertROCK2 (ROCK2 Human)
UseGateway CloningAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLJM60-FLAG-SLC38A9.1
Plasmid#71858Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmGFP11C-Tau
Plasmid#71434PurposeSplit GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTau (0N4R) (MAPT Human)
TagsGFP (213-228)ExpressionMammalianAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TetO-FUW-NfiA
Plasmid#64901PurposeInducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.DepositorAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
S1PR1-Tango
Plasmid#66496PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertS1PR1 (S1PR1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Lifeact-EGFP
Plasmid#67303PurposeFluorescent indicator for F-actinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeact-EGFP
ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE-60 roGFP2-His
Plasmid#65046PurposeBacterial expression of His-tagged roGFP2DepositorInsertroGFP2
TagsHisExpressionBacterialMutationsee sequenceAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEM5Z(+)-EGFP
Plasmid#65206PurposeEGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
ExpressionBacterial and MammalianMutationRestriction enzyme recognition sites added either…PromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ii-Str_TNF-SBP-EGFP
Plasmid#65280Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTNF (TNF Human)
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFRT-TODestFLAGHA_RNAseH1
Plasmid#65782Purposeexpresses FLAGHA-tagged RNAsH1 in mammalian cellsDepositorInsertRNaseH1 (RNASEH1 Zebrafish, Human)
TagsFLAG HA and V5ExpressionMammalianPromoterCMV, 2x TetAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-HA-Nrxn1beta AS4(+)
Plasmid#59410PurposeExpression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNrxn1beta AS4(+) (Nrxn1 Mouse)
TagsHAExpressionMammalianPromoterCAGAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCYC1m_yeGFP
Plasmid#64389Purposeencodes yeast enhanced GFP under control of minimal pCYC1 promoterDepositorPublicationInsertyeast enhanced GFP
ExpressionYeastPromoterpCYC1Available sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SmoM2 (W535L)-pcw107-V5
Plasmid#64628Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTpPuc3
Plasmid#62864PurposeEpisome vector for Thalassiosira pseudonana (pUC-based)DepositorTypeEmpty backboneUseSynthetic Biology; Episomal vector for thalassios…Available sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only