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169,450 results

Showing: 10821 - 10840 of 169450 results

  5. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

    Plasmid
    #234951
    Purpose
    Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    234951-plasmid-map-sequence-id-467430

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  13. AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR

    Plasmid
    #191795
    Purpose
    vector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    A134V, A140V, A141V, A143V
    Promoter
    EFS, hU6
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191795-plasmid-map-sequence-id-375580

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  14. pLB

    Plasmid
    #11619
    Purpose
    pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.
    Depositor
    Stephan Kissler
    Article
    Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    mouse U6
    Available Since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11619-plasmid-map-sequence-id-274493

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  15. pCAG-EGxxFP

    Plasmid
    #50716
    Purpose
    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Article
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Insert
    split EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    50716-plasmid-map-sequence-id-447281

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  16. pBJ6

    Plasmid
    #167135
    Purpose
    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Expression
    Bacterial
    Promoter
    Kanamycin promoter
    Available Since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167135-plasmid-map-sequence-id-330485

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  19. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212963
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_GFP targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212963-plasmid-map-sequence-id-422499

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  20. TFORF2546

    Plasmid
    #144209
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CEBPA (CEBPA Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144209-plasmid-map-sequence-id-404084

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Showing: 10821 - 10840 of 169450 results