167,513 results

1. 7TGP

   Plasmid

   #24305

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-eGFP // SV40-PuroR
   

   Use

   Lentiviral

   Mutation

   EGFP is GFP with S65T and F64L mutations

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGDP1 fosA

   Plasmid

   #112889

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   fosA
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Mito-B

   Plasmid

   #209864

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Tags

   Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Sst44-GFP

   Plasmid

   #172310

   Purpose

   SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

   Depositor

   Michael Greenberg

   Article

   Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

   Insert

   Sst44
   

   Use

   AAV

   Promoter

   pB-Globin

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30a-AlkB-D135S

   Plasmid

   #79051

   Purpose

   Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBG100-TCPTP

   Plasmid

   #33365

   Depositor

   Ambra Pozzi

   Article

   Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

   Insert

   TCPTP-45 (PTPN2 Human)
   

   Tags

   6xHIS - cleavable

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

7. GCH1_pLX307

   Plasmid

   #98336

   Purpose

   Lentiviral expression of GCH1

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   GCH1 (GCH1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO-GAlphasS-RLuc8

   Plasmid

   #140980

   Purpose

   Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphasS-RLuc8
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKDsgRNA-pgaC

   Plasmid

   #154919

   Purpose

   aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting pgaC
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid

    #212022

    Purpose

    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GianCreg (GOLGB1 Human)
    

    Tags

    td5StayGold(c4)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmScarlet3-H_I3

    Plasmid

    #239343

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Tags

    mScarlet3-H (a.k.a. mYongHong)

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787)

    Plasmid

    #191726

    Purpose

    AI plasmid ALIAS: AiP12787. Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression

    Depositor

    Jonathan Ting, Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    SYFP2
    

    Use

    AAV

    Mutation

    none

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-iLACCO1.2

    Plasmid

    #218823

    Purpose

    Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1.2
    

    Expression

    Mammalian

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid

    #60224

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Promoter

    EFS, No promoter, and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-GAL4

    Plasmid

    #24345

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    GAL4
    

    Expression

    Mammalian

    Available Since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bxb1-GA_AIO-TetOn_mScarlet_Divergent

    Plasmid

    #229791

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

    Plasmid

    #159816

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-CAG-empty-pgk-hph

    Plasmid

    #48753

    Purpose

    PiggyBac backbone, CAG promoter, Hygromycin selection

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Type

    Empty backbone

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pDK521

    Plasmid

    #134204

    Purpose

    Baculovirus expresion of Astrin, LC8, and SKAP

    Depositor

    Iain Cheeseman

    Article

    Kern et al Elife. 2017 Aug 25;6. pii: 26866. doi: 1

    Insert

    Astrin 1-693-sGFP-His BroAstrin and LC8 and AltSKAP in pACE1
    

    Expression

    Insect

    Available Since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 791 p3E-2A-pCS2MCSpA v2

    Plasmid

    #195973

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Kristen Kwan, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-multiple cloning site
    

    Use

    Other

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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