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Repurposing CRISPR-Cas12b for mammalian genome engineering.
Teng F, Cui T, Feng G, Guo L, Xu K, Gao Q, Li T, Li J, Zhou Q, Li W
Cell Discov. 2018 Nov 27;4:63. doi: 10.1038/s41421-018-0069-3. eCollection 2018.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
121945pCAG-AaCas12b-2AeGFPMammalian expression, Genome editing
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121946pCAG-AkCas12b-2AeGFPMammalian expression, Genome editing
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121947pCAG-AmCas12b-2AeGFPMammalian expression, Genome editing
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121948pCAG-BsCas12b-2AeGFPMammalian expression, Genome editing
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121949BPK2014-AaCas12bE. coli expression, protein purification
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121950BPK2013-AkCas12bE. coli expression, protein purification
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121951BPK2014-AmCas12bE. coli expression, protein purification
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121952BPK2013-BsCas12bE. coli expression, protein purification
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121953pUC19-U6-AagRNAMammalian expression, Genome editing, gRNA scaffold
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121954pUC19-U6-AasgRNAMammalian expression, Genome editing, gRNA scaffold
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121955pUC19-U6-AksgRNAMammalian expression, Genome editing, gRNA scaffold
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121956pUC19-U6-AmsgRNAMammalian expression, Genome editing, gRNA scaffold
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121957pUC19-U6-BssgRNAMammalian expression, Genome editing, gRNA scaffold
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121958pUC19-U6-AasgRNA3.8Mammalian expression, Genome editing, gRNA scaffold
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121959pUC19-U6-AasgRNA3.8.3Mammalian expression, Transcription regulation, gRNA scaffold
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121961pCAG-AaCas12b(R911A)-2AeGFPMammalian expression, Transcription regulation, deactivated AaCas12b
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121962pCAG-AaCas12b(D977A)-2AeGFPMammalian expression, Transcription regulation, deactivated AaCas12b
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121963pCAG-MCP-VP64-2AmScarletMammalian expression, Transcription regulation module
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121964pCAG-MCP-VPR-2AmScarletMammalian expression, Transcription regulation module
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