181,923 results
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Plasmid#167579PurposeExpress the ATP sensor GRAB_ATP1.0 in astrocytesDepositorAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pTTCC
Plasmid#164264PurposeExpresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilusDepositorInsertsCas9
gRNA scaffold
ExpressionBacterialPromoterPcel and trcAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEEV2/11
Plasmid#166888PurposeHelper/packaging plasmid for production of AAV2/11 pseudotype vectorDepositorInsertAAV2 rep/AAV11 cap
UseAAVPromoterAAV2 p5Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS31-cCARLIN
Plasmid#160928PurposeContains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCARLIN gRNAs and CAG-GFP-CARLIN array
UseMouse TargetingExpressionMammalianPromoterU6 promoter for gRNAs; CAG promoter for GFPAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SFPQ
Plasmid#166960PurposeExpresses FLAG-tagged SFPQ protein in pcDNA3.1DepositorAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSynapsin1-axon-GCaMP6f (AAV1)
Viral prep#112000-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-GCaMP6f (#112000). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6f plasmid DNA. Synapsin-driven, axon-targeted GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-Opti
Plasmid#163126PurposeLentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.DepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTet-O-ASCL1-T2A-PuroR
Plasmid#162345PurposeLentiviral expression of ASCL1 under the control of the TetON promoterDepositorAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#161927PurposeDonor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSurface marker and Citrine repression reporter
UseSynthetic BiologyExpressionMammalianPromoterpEFAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16
Plasmid#163029PurposeFLiCRE TF for neuronal expressionDepositorInsertNrxn3b-MKII-hLOV1-TEVcs-tTA
UseAAVExpressionMammalianPromoterSynapsinAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hACE2-hygro
Plasmid#161758PurposeExpresses human ACE2 in mammalian cellsDepositorAvailable sinceNov. 5, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pEGBacmam-NGS Wnt-Fc
Plasmid#159629PurposeExpress Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid researchDepositorInsertFzd7/8 subtype NGS Wnt Fc
TagsFc tag and His tagExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Sec61B
Plasmid#141152PurposeFluorescent labeling of ER membrane in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec61 beta (SEC61B Human)
TagsSNAP tagExpressionMammalianMutationL79M on Sec61BPromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MAC-NRP1
Plasmid#158384PurposeMAC-tagged gene expressionDepositorAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pT3TS-RfxCas13d-NLS-HA
Plasmid#141321PurposePlasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRfx-Cas13d (NLS-RfxCas13d-NLS)
UseCRISPRTagsHA and NLSAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-dCas13-inactive M3nls
Plasmid#157854PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
ExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395AAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-PspCas13b-gRNA-Actb1216
Plasmid#155368PurposePspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPspCas13b gRNA targeting Actb 1216
UseCRISPRExpressionMammalianAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-Tet
Plasmid#138086PurposeEmpty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particlesDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterminimal CMV promoter with heptamerized tet‐operat…Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
3XFLAG-VP64-SadCas9-NLS-VP64
Plasmid#135338Purpose3xFLAG 2xVP64 SadCas9 for single guide cloningDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSadCas9
Tags3xFLAG-VP64-SV40 NLS and NLS-VP64ExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTol1-UAS:CoChR-tdTomato
Plasmid#124233PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomatoDepositorInsertCoChR-tdTomato
UseZebrafish expressionTagstdTomatoPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by