163,268 results

1. pCDNA6A-EGFR WT

   Plasmid

   #42665

   Depositor

   Mien-Chie Hung

   Article

   Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG mito-mEos2

   Plasmid

   #168515

   Purpose

   Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

   Depositor

   Franck Polleux

   Article

   Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

   Insert

   mito-mEos2
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/Ndi-1/myc-His

   Plasmid

   #127503

   Purpose

   Expresses myc-tagged yeast Ndi-1 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   Ndi-1 (NDI1 Budding Yeast)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK287 (mAID-Hygro)

   Plasmid

   #72825

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Hygro
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. cEF.tk-GFP

   Plasmid

   #33308

   Depositor

   Linzhao Cheng

   Article

   Pomper et al Cell Res. 2009 Mar;19(3):370-9.

   Insert

   HSV-TK/GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-nEF-Con/Fon-iC++-EYFP (AAV8)

   Viral Prep

   #137155-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-iC++-EYFP (#137155). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-iC++-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Cre and Flp-dependent)

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

7. pKDsgRNA-ack

   Plasmid

   #62654

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPRv2R

   Plasmid

   #167932

   Purpose

   lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. iCas

    Plasmid

    #84232

    Purpose

    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

    Depositor

    Meng How Tan

    Article

    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    2A-OFP co-expression and ERT2-ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-sfGFP

    Plasmid

    #125747

    Purpose

    sfGFP without iCreERT2 fusion

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF5B-FRT-cilia-APEX

    Plasmid

    #73186

    Purpose

    Expresses cilia-APEX in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    cilia-APEX (Nphp3 Mouse, Glycine max)
    

    Use

    Tags

    internal GFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-CAG-hChR2-H134R-tdTomato (AAV Retrograde)

    Viral Prep

    #28017-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from AAV-CAG-hChR2-H134R-tdTomato (#28017). In addition to the viral particles, you will also receive purified AAV-CAG-hChR2-H134R-tdTomato plasmid DNA. Humanized channelrhodopsin H134R mutant fused to tdTomato, under the control of the CAG promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Promoter

    CAG

    Tags

    tdTomato

    Available since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

14. Col1A2:TFPnls-T2a-mErt-Cre-Ert

    Plasmid

    #111151

    Purpose

    Fibroblasts specific inducible Cre

    Depositor

    Elly Tanaka

    Insert

    TFPnls-T2a-mErt-Cre-Ert
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    wt

    Promoter

    Col1A2

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. ARR3tk-eGFP/SV40-mCherry

    Plasmid

    #132360

    Purpose

    To measure transcriptional activity of Androgen Receptor

    Depositor

    Charles Sawyers

    Article

    Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

    Insert

    AR responsive elements (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV/TO eGFP Puro (w159-1)

    Plasmid

    #17481

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. HAT1

    Plasmid

    #25239

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HAT1_05 (HAT1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pUK21

    Plasmid

    #49788

    Purpose

    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Halo-Rab6A

    Plasmid

    #190171

    Purpose

    Expresses HaloTag-Rab6A in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    HaloTag-Rab6A
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2xP53_RE::FLuc

    Plasmid

    #178316

    Purpose

    P53 Firefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    P53 FLuc Reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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