167,514 results

1. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiGuide-Hygro-eGFP

   Plasmid

   #99375

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET31b-T7-Spinach2

   Plasmid

   #79783

   Purpose

   Expresses Spinach2 under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   T7-tRNA-Spinach2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-hSyn-hChR2(H134R)-mCherry

   Plasmid

   #26976

   Purpose

   AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

   Depositor

   Karl Deisseroth

   Has Service

   AAV5, AAV8, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   hSyn

   Available Since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-SYN-eGFP-Kindlin 1

   Plasmid

   #203733

   Purpose

   AAV vector plasmid expressing mouse kindlin-1 fused to eGFP under the human synapsin (SYN) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Insert

   Fermt1 (Fermt1 Mouse)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   Human synapsin promoter

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-PM-TORCAR

   Plasmid

   #64930

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   Lyn-TORCAR (EIF4EBP1 Synthetic, Human)
   

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2]

   Plasmid

   #195488

   Purpose

   AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-tdTomato-ER2
   

   Use

   AAV

   Tags

   ER2, KGC, and tdTomato

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CAG

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

   Plasmid

   #162381

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   CAG

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-FRB

    Plasmid

    #25919

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    FRB (MTOR Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid

    #154786

    Purpose

    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

    Depositor

    Robert Singer

    Article

    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

    Insert

    Adenosine deaminse (Adar Synthetic, Fly)
    

    Use

    Lentiviral

    Tags

    GFP, V5, and mCherry

    Expression

    Mammalian

    Mutation

    Synonymized serine to prevent autoediting of ADAR…

    Promoter

    UBC

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDB179-TurboPETase

    Plasmid

    #218693

    Purpose

    Encodes codon optimized TurboPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TurboPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGLO-Blue

    Plasmid

    #220782

    Purpose

    Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)

    Depositor

    Angela Leu

    Insert

    blue chromoprotein (aeBlue)
    

    Tags

    8xHis

    Expression

    Bacterial

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FKBP-CD28TMD-NTEVp(H75S)

    Plasmid

    #137828

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75S)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Serine in NTEVp fragment

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI

    Plasmid

    #205162

    Purpose

    express scFv-GCN4-HAtag-msfGFP(V206K)-GBI

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    scFv-GCN4-HAtag-msfGFP(V206K)-GBI
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV500 empty vector

    Plasmid

    #33348

    Depositor

    Charles Vinson

    Article

    Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAK_EF1a_CasRx_Hygro

    Plasmid

    #134840

    Purpose

    Lentiviral expression of CasRx T2A Hygromycin

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR and Lentiviral

    Tags

    T2A Hygromycin

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAct-dCBEevoCDA1

    Plasmid

    #195515

    Purpose

    Ubiquitous expression of dCBEevoCDA1 in Drosophila

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    dCBE-evoCDA1
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    act5C

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFGAMS-mEos2

    Plasmid

    #105128

    Purpose

    Expresses human FGAMS-mEos2 fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    French et al Science. 2016 Feb 12;351(6274):733-7. do

    Insert

    PFAS (PFAS Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E_he1a:mCherry

    Plasmid

    #113881

    Purpose

    p3E entry vector for Three Multisite Gateway

    Depositor

    Marnie Halpern

    Insert

    he1a (he1.1 Zebrafish)
    

    Use

    Zebrafish danio expression

    Tags

    mCherry

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge