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(Plasmid #124233)


Item Catalog # Description Quantity Price (USD)
Plasmid 124233 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    from Harold Burgess lab (Addgene Plasmid #62215)
  • Backbone size w/o insert (bp) 4548
  • Total vector size (bp) 6860
  • Modifications to backbone
    NcoI linearization before insert cloning
  • Vector type
    Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Chloromonas oogama
  • Insert Size (bp)
  • GenBank ID
  • Promoter 14xUAS-E1b
  • Tag / Fusion Protein
    • tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TCA GTC GTC CGT TTG TTC CG
  • 3′ sequencing primer GAG CAG CAA AGC CAT GTA GC
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pAAV-Syn-CoChR-GFP (Addgene Plasmid #59070). Independent optical excitation of distinct neural populations. Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, Boyden ES. Nat Methods. 2014
  • Article Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato. In addition to the opsin gene, it contains tol1 arms, 14xUAS-E1b promoter, ubiquitin C intron, and ocean pout antifreeze protein terminator. Co-inject with Tol1 (not Tol2) transposase mRNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol1-UAS:CoChR-tdTomato was a gift from Isaac Bianco (Addgene plasmid # 124233 ; ; RRID:Addgene_124233)
  • For your References section:

    A calibrated optogenetic toolbox of stable zebrafish opsin lines. Antinucci P, Dumitrescu A, Deleuze C, Morley HJ, Leung K, Hagley T, Kubo F, Baier H, Bianco IH, Wyart C. Elife. 2020 Mar 27;9. pii: 54937. doi: 10.7554/eLife.54937. 10.7554/eLife.54937 PubMed 32216873