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Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens.
Black JB, McCutcheon SR, Dube S, Barrera A, Klann TS, Rice GA, Adkar SS, Soderling SH, Reddy TE, Gersbach CA
Cell Rep. 2020 Dec 1;33(9):108460. doi: 10.1016/j.celrep.2020.108460.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
162333pLV_hUbC-dSpCas9-2xVP64-T2A-BSDLentiviral expression of dSpCas9-2xVP64 with blasticidin resistance
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162334pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroRLentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance
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162335pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroRLentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker
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162336pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFPLentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker
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162337pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFPLentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker
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162338pTet-O-NEUROD1-T2A-PuroRLentiviral expression of NEUROD1 under the control of the TetON promoter
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162339pTet-O-NEUROG1-T2A-PuroRLentiviral expression of NEUROG1 under the control of the TetON promoter
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162341pTet-O-NEUROG3-T2A-PuroRLentiviral expression of NEUROG3 under the control of the TetON promoter
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162342pTet-O-ATOH1-T2A-PuroRLentiviral expression of ATOH1 under the control of the TetON promoter
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162343pTet-O-ATOH7-T2A-PuroRLentiviral expression of ATOH7 under the control of the TetON promoter
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162345pTet-O-ASCL1-T2A-PuroRLentiviral expression of ASCL1 under the control of the TetON promoter
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162346pTet-O-KLF7-T2A-PuroRLentiviral expression of KLF7 under the control of the TetON promoter
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162347pTet-O-OVOL1-T2A-PuroRLentiviral expression of OVOL1 under the control of the TetON promoter
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162348pTet-O-E2F7-T2A-PuroRLentiviral expression of E2F7 under the control of the TetON promoter
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162349pTet-O-RUNX3-T2A-PuroRLentiviral expression of RUNX3 under the control of the TetON promoter
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162350pTet-O-LHX8-T2A-PuroRLentiviral expression of LHX8 under the control of the TetON promoter
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