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Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens.

Black JB, McCutcheon SR, Dube S, Barrera A, Klann TS, Rice GA, Adkar SS, Soderling SH, Reddy TE, Gersbach CA
Cell Rep. 2020 Dec 1;33(9):108460. doi: 10.1016/j.celrep.2020.108460. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
162333pLV_hUbC-dSpCas9-2xVP64-T2A-BSDLentiviral expression of dSpCas9-2xVP64 with blasticidin resistance
162334pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroRLentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance
162335pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroRLentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker
162336pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFPLentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker
162337pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFPLentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker
162338pTet-O-NEUROD1-T2A-PuroRLentiviral expression of NEUROD1 under the control of the TetON promoter
162339pTet-O-NEUROG1-T2A-PuroRLentiviral expression of NEUROG1 under the control of the TetON promoter
162341pTet-O-NEUROG3-T2A-PuroRLentiviral expression of NEUROG3 under the control of the TetON promoter
162342pTet-O-ATOH1-T2A-PuroRLentiviral expression of ATOH1 under the control of the TetON promoter
162343pTet-O-ATOH7-T2A-PuroRLentiviral expression of ATOH7 under the control of the TetON promoter
162345pTet-O-ASCL1-T2A-PuroRLentiviral expression of ASCL1 under the control of the TetON promoter
162346pTet-O-KLF7-T2A-PuroRLentiviral expression of KLF7 under the control of the TetON promoter
162347pTet-O-OVOL1-T2A-PuroRLentiviral expression of OVOL1 under the control of the TetON promoter
162348pTet-O-E2F7-T2A-PuroRLentiviral expression of E2F7 under the control of the TetON promoter
162349pTet-O-RUNX3-T2A-PuroRLentiviral expression of RUNX3 under the control of the TetON promoter
162350pTet-O-LHX8-T2A-PuroRLentiviral expression of LHX8 under the control of the TetON promoter

Antibodies from Article