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Addgene

pTet-O-ASCL1-T2A-PuroR
(Plasmid #162345)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162345 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Tet-O-FUW
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ASCL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    708
  • Entrez Gene
    ASCL1 (a.k.a. ASH1, HASH1, MASH1, bHLHa46)
  • Promoter Tet-ON
  • Tag / Fusion Protein
    • T2A-PuroR (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was modifed from a gift from Marius Wernig (Addgene plasmid # 52047)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTet-O-ASCL1-T2A-PuroR was a gift from Charles Gersbach (Addgene plasmid # 162345 ; http://n2t.net/addgene:162345 ; RRID:Addgene_162345)
  • For your References section:

    Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens. Black JB, McCutcheon SR, Dube S, Barrera A, Klann TS, Rice GA, Adkar SS, Soderling SH, Reddy TE, Gersbach CA. Cell Rep. 2020 Dec 1;33(9):108460. doi: 10.1016/j.celrep.2020.108460. 10.1016/j.celrep.2020.108460 PubMed 33264623