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pLV_hUbC-dSpCas9-2xVP64-T2A-BSD
(Plasmid #162333)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 162333 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FUGW
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dSpCas9-2xVP64
  • Species
    S. pyogenes
  • Mutation
    D10A and H840A
  • Promoter hUbC
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV_hUbC-dSpCas9-2xVP64-T2A-BSD was a gift from Charles Gersbach (Addgene plasmid # 162333 ; http://n2t.net/addgene:162333 ; RRID:Addgene_162333)
  • For your References section:

    Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens. Black JB, McCutcheon SR, Dube S, Barrera A, Klann TS, Rice GA, Adkar SS, Soderling SH, Reddy TE, Gersbach CA. Cell Rep. 2020 Dec 1;33(9):108460. doi: 10.1016/j.celrep.2020.108460. 10.1016/j.celrep.2020.108460 PubMed 33264623