PurposeDHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||69534||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 12561
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)6138
- Promoter CAG
/ Fusion Proteins
- DHFR Destabilised Domain (N terminal on insert)
- C-term T2A-EGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pCAG-F GCAACGTGCTGGTTATTGTG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bydCas9 Mutated from pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A), Addgene Plasmid #42335; DHFR cloned from pBMN DHFR(DD)-YFP, Addgene Plasmid #29325
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CAG-DDdCas9VP192-T2A-EGFP-ires-puro was a gift from Timo Otonkoski (Addgene plasmid # 69534 ; http://n2t.net/addgene:69534 ; RRID:Addgene_69534)
For your References section:Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. 10.1016/j.stemcr.2015.08.001 PubMed 26352799