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Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation.
Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T
Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
69534CAG-DDdCas9VP192-T2A-EGFP-ires-puroDHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
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69535pCXLE-dCas9VP192-T2A-EGFP-shP53Episomal plasmid encoding dCas9VP192 and p53 shRNA
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69536pCXLE-dCas9VP192-T2A-EGFPEpisomal plasmid encoding dCas9VP192
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69537GG-EBNA-OCT4Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter
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69538GG-destDestination plasmid for gRNA concatenation Golden Gate cloning
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