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pLentiV2-dCas9-VP64
(Plasmid #141104)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 141104 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    entiSAM v2 (Puro) addgene#92062
  • Backbone size (bp) 15524
  • Modifications to backbone
    The gRNA cloning site was replaced with the one from lentiCRISPR v2 (addgene# 52961)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiV2-dCas9-VP64 was a gift from Igor Ulitsky (Addgene plasmid # 141104 ; http://n2t.net/addgene:141104 ; RRID:Addgene_141104)