179,539 results
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Plasmid#107229PurposeTomato expressing plasmidDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pEJS613_pTetR-P2A-BFPnls/sgTelo
Plasmid#108649PurposeTelomere-targeting Spy sgRNA under U6 promoterDepositorInsertsTelomere-targeting sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianPromoterU6 and hPGKAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP‐RNASEH2B
Plasmid#108697PurposeFor expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRNASEH2B (RNASEH2B Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-HA-UBE3A-IRES-Puro
Plasmid#107349PurposeExpresses HA tagged UBE3A in mammalian cells.DepositorAvailable sinceApril 23, 2018AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
EMMA toolkit
Plasmid kit#1000000119PurposeCollection of Golden Gate vectors and standardized genetic parts for the construction mammalian expression plasmids.DepositorApplicationCloning and Synthetic BiologyVector typeMammalian ExpressionCloning typeGolden GateAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVTREGCaMP6f
Plasmid#97410PurposeAmplified expression of GCaMP6f in combination with TET driverDepositorInsertGCaMP6f
UseAAVExpressionMammalianPromoterTRE3Available sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_Thy1StTA
Plasmid#97411PurposeTET driver for amplified expression in cortical neuronsDepositorInserttTA
UseAAVExpressionMammalianPromotermouse thy1PSsAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.Flex.GCaMP6s.WPRE.SV40
Plasmid#100845PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas serviceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-Cas9-162A
Plasmid#84918Purposefor in vitro transcription of Cas9 with polyA tail encodedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
TagsFlag, NLS, and polyA tailExpressionMammalianPromoterCAGAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G9A[SET]-dCas9
Plasmid#100089PurposeCatalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertG9A (EHMT2 Human)
UseCRISPRTags3XFLag-NLS-G9A[SET]-dCas9-NLSExpressionMammalianMutationG9A[SET] includes aa 829-1209PromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT EGFP-miniAID
Plasmid#101714PurposeFor amplification of a EGFP-miniAID for N-terminal taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-miniAID
TagsEGFP-miniAIDExpressionMammalianAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-ihSyn1-DIO-tTA
Plasmid#99121PurposeAn AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTADepositorHas serviceAAV1InsertDIO-tTA
UseAAVExpressionMammalianMutation*(see below)PromoterihSyn1Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
YIp204-PADH1-atTIR1-9myc
Plasmid#99532PurposeS. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5
Plasmid#97044PurposeExpresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.DepositorInsertLigand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
UseLentiviralExpressionMammalianPromoterTet onAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-IGHG2
Plasmid#99576PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG2 isotypeDepositorTypeEmpty backboneExpressionMammalianPromoterhCMV IE1 gene promoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
3xnls-mTurquoise2
Plasmid#98817PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xNLS
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-Nanoluc-ccdB
Plasmid#87070PurposeMammalian Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferaseDepositorTypeEmpty backboneTagsNanoluc luciferaseExpressionMammalianAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOD_A0101
Plasmid#90998PurposeModule A, Promoter: 35S, Gene: AtCas9, Terminator: HSPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAtCas9
UseCRISPRPromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir
Plasmid#90009PurposeSplit-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBPDepositorInsertsFKBP-linker-Myc-NBirA*
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBHRSF184
Plasmid#89482PurposeExpress asparaginyl endopeptidase 1 from O. affinis (OaAEP1)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
TagsSmt3ExpressionBacterialPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by