|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10815||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3176
Vector typeMammalian Expression, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePKD1 RNAi
SpeciesH. sapiens (human)
Insert Size (bp)64
Entrez GenePRKD1 (a.k.a. CHDED, PKC-MU, PKCM, PKD, PRKCM)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCATG CTGTGGGGGC TGGTACTTCA AGAGAGTACC AGCCCCCACA GCATTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAATGCTG TGGGGGCTGG TACTCTCTTG AAGTACCAGC CCCCACAGCA TGGG-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSUPER.PKD1.RNAi was a gift from Alex Toker (Addgene plasmid # 10815 ; http://n2t.net/addgene:10815 ; RRID:Addgene_10815)
For your References section:Protein kinase D mediates a stress-induced NF-kappaB activation and survival pathway. Storz P, Toker A. EMBO J. 2003 Jan 2. 22(1):109-20. 10.1093/emboj/cdg009 PubMed 12505989