Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10818||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3176
Vector typeMammalian Expression, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePKD3 RNAi
SpeciesH. sapiens (human)
Insert Size (bp)64
Entrez GenePRKD3 (a.k.a. EPK2, PKC-NU, PKD3, PRKCN, nPKC-NU)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCGTC CTAAGACGGG ACTCTCTTCA AGAGAGAGAG TCCCGTCTTA GGACTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAGTCCTA AGACGGGACT CTCTCTCTTG AAGAGAGTCC CGTCTTAGGA CGGG-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSUPER.PKD3.RNAi was a gift from Alex Toker (Addgene plasmid # 10818 ; http://n2t.net/addgene:10818 ; RRID:Addgene_10818)
For your References section:Protein kinase D regulates vesicular transport by phosphorylating and activating phosphatidylinositol-4 kinase IIIbeta at the Golgi complex. Hausser A, Storz P, Martens S, Link G, Toker A, Pfizenmaier K. Nat Cell Biol. 2005 Sep . 7(9):880-6. 10.1038/ncb1289 PubMed 16100512