Engineering RNA export for measurement and manipulation of living cells.
Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB
Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013. PubMed Article
Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB
Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205520 | pFH2.1_mCherry | Express mCherry (without export tag) |
| 205521 | pFH2.2_mCherry-MS2x12 | Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer) |
| 205522 | pFH2.4_mCherry-MMLV_Psi | Express mCherry-Psi (export tag consisting of MMLV Psi) |
| 205523 | pFH2.7_Gag-dZF2-MCP-P2A-eGFP | Express HIV Gag-delta-ZF2-MCP-P2A-eGFP |
| 205524 | pFH2.9_MMLV_Gag-P2A-eGFP | Express MMLV Gag-P2A-eGFP |
| 205525 | pFH2.10_Gag-MCP-P2A-eGFP | Express HIV Gag-MCP-P2A-eGFP |
| 205526 | pFH2.11_Gag-MCP_ZF2-P2A-eGFP | Express HIV Gag-MCP_ZF2-P2A-eGFP |
| 205527 | pFH2.12_GagZip-MCP-P2A-eGFP | Express HIV GagZip-MCP-P2A-eGFP |
| 205528 | pFH2.13_GagZip-MCP_Zip-P2A-eGFP | Express HIV GagZip-MCP_Zip-P2A-eGFP |
| 205529 | pFH2.14_GagZip-dp1dp6-MCP-P2A-eGFP | Express HIV GagZip-dp1dp6-MCP-P2A-eGFP |
| 205531 | pFH2.16_MiniGagZip-MCP_Zip-P2A-eGFP | Express HIV MiniGagZip-MCP_Zip-P2A-eGFP |
| 205532 | pFH2.17_GagZip-MCP-dpol-P2A-eGFP | Express HIV GagZip-MCP-dpol-P2A-eGFP |
| 205533 | pFH2.18_TagBFP | Express TagBFP |
| 205534 | pFH2.19_mCherry-MS2x2 | Express mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer) |
| 205535 | pFH2.20_mCherry-MS2x4 | Express mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer) |
| 205536 | pFH2.21_mCherry-MS2x6 | Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer) |
| 205537 | pFH2.22_mCherry-MS2x8 | Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer) |
| 205538 | pFH2.30_Gag-MCP-dpol-T2A-eGFP | Express HIV Gag-MCP-dpol-T2A-eGFP |
| 205539 | pFH2.32_LV-mCherry-MS2x8-BCS | Express mCherry-MS2x8 with barcode |
| 205540 | pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast | Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker |
| 205541 | pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast | Express EPN1-T2A-eGFP and blasticidin resistance marker |
| 205542 | pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast | Express EPN1-MCP-T2A-eGFP and blasticidin resistance marker |
| 205543 | pFH2.98_PB-EPN1-MCP_Myc-T2A-eGFP-PGK-Blast | Express EPN1-MCP-Myc-T2A-eGFP and blasticidin resistance marker |
| 205544 | pFH2.99_PB-EPN1-MCP_I-T2A-eGFP-PGK-Blast | Express EPN1-MCP-I-T2A-eGFP and blasticidin resistance marker |
| 205545 | pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast | Express EPN11-T2A-eGFP and blasticidin resistance marker |
| 205546 | pFH2.101_PB-EPN11-MCP-T2A-eGFP-PGK-Blast | Express EPN11-MCP-T2A-eGFP and blasticidin resistance marker |
| 205547 | pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast | Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker |
| 205548 | pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast | Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker |
| 205549 | pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast | Express EPN24-T2A-eGFP and blasticidin resistance marker |
| 205550 | pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast | Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker |
| 205551 | pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast | Express EPN24-MCP-I-T2A-eGFP and blasticidin resistance marker |
| 205552 | pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast | Express EPN24-MCP-p6-T2A-eGFP and blasticidin resistance marker |
| 205553 | pFH2.155_LV-Puro-T2A-mTagBFP2 | Express puromycin resistance marker and mTagBFP2 |
| 205554 | pFH2.156_LV-Zeo | Express zeocin resistance marker |
| 205555 | pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast | Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker |
| 205556 | pFH2.214_mCherry-PP7x12 | Express mCherry-PP7x12 (export tag consisting of 12 tandem repeats of PP7 stem-loop aptamer) |
| 205557 | pFH2.217_PB-EPN24-PCP-IRES-eGFP-PGK-Blast | Express EPN24-PCP-IRES-eGFP and blasticidin resistance marker |
| 205558 | pFH3.2_mTagBFP2-MS2x12 | Express mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer) |
| 205559 | pFH3.3_Cre-MS2x12 | Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer) |
| 205560 | pFH3.29_Cre | Express Cre (without export tag) |
| 205561 | pJAM1.16_PB-Cx43_S368A-PGK-Blast | Express human Cx43 with S368A mutation |
| 205562 | pJAM1.17_PB-STEAP3-PGK-Blast | Express human STEAP3 |
| 205563 | pJAM1.18_PB-SDC4-PGK-Blast | Express human SDC4 |
| 205564 | pJAM1.19_PB-CIT-PGK-Blast | Express human CIT |
| 205565 | pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast | Express human NEDD4L (delta C2 isoform) |
| 205566 | pJAM1.21_PB-UGCG-PGK-Blast | Express human UGCG |