169,099 results

1. pENTR4_G3BP2

   Plasmid

   #127105

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   G3BP2 (G3BP2 Human)
   

   Use

   Gateway shuttling vector

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. shCTNNB1 # 2

   Plasmid

   #42544

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. PVS10

   Plasmid

   #104398

   Purpose

   polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

   Plasmid

   #205631

   Purpose

   Expression of TnpBmax in mammalian cells

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   ISDra2-TnpB optimized for mammalian expression
   

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCMV_DENV2_PrME

   Plasmid

   #207264

   Purpose

   Mammalian expression of Dengue virus 2 PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   DENV2_PrME (POLY Dengue virus 2)
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF5B-FRT-GFP-αTAT1[D157N]

   Plasmid

   #27100

   Depositor

   Maxence Nachury

   Article

   Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

   Insert

   αTubulin K40 acetyltransferase (ATAT1 Human)
   

   Tags

   GFP and S-tag

   Expression

   Mammalian

   Mutation

   D157N

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. MEK1-DD-V5

   Plasmid

   #217298

   Purpose

   Lentivirus vector for expression of MEK1 S218D/S222D

   Depositor

   Channing Der

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   S218D/S222D

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rogi1-CMV-LAMB3-T2A-GFP-bGH

   Plasmid

   #179864

   Purpose

   Targets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFP

   Depositor

   George Church

   Article

   Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

   Insert

   LAMB3-T2A-GFP
   

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOSIP-KO (KanR, 186)

   Plasmid

   #45985

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. QQR1 variant

    Plasmid

    #138564

    Purpose

    Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    QQR1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-MBP-TEVpCS-ADGRB1 CTF-FLAG

    Plasmid

    #217681

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB1 (ADGRB1 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-926

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTi4.0_SpdCas9_pmcDA1

    Plasmid

    #204613

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)

    Plasmid

    #203909

    Purpose

    Encodes wild type Gal4-VP64 transcription factor in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    Gal4-VP64-IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. tol2-mpeg1-mcherry

    Plasmid

    #58935

    Purpose

    labels macrophages with mcherry

    Depositor

    Anna Huttenlocher

    Insert

    mpeg1-mcherry
    

    Promoter

    mpeg1

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-EGFP-C-IFT88

    Plasmid

    #218733

    Purpose

    Expresses N-terminally EGFP-tagged IFT88 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT88 (IFT88 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

    Plasmid

    #172982

    Purpose

    control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    control sgRNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. R777-E227 Hs.RASA1

    Plasmid

    #70511

    Purpose

    Gateway ORF clone of human RASA1 [NM_002890.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASA1 (RASA1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMGF182

    Plasmid

    #97006

    Purpose

    Expresses GFP-CHMP7 in mammalian cells under a crippled promoter

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    CHMP7 (CHMP7 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV delta 5

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEG BacMam C term 3C eGFP StrepII

    Plasmid

    #160686

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Tags

    3C eGFP StrepII

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA6.2 EmGFP hAQP4(M23)

    Plasmid

    #126464

    Purpose

    Expresses human AQP4 (isoform M23) as an EmGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

    Insert

    Aquaporin 4 (AQP4 Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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