pcDNA Flag PGC1 T177A S538A
(Plasmid #18093)

• Depositing Lab: Bruce Spiegelman
• Publication: Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 104(29):12017-22.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PGC-1a
• Alt name: PPAR gamma coactivator 1 alpha
• Alt name: PGC1a
• Alt name: PGC1 alpha
• Species: M. musculus (mouse)
• Insert Size (bp): 2400
• Mutation: T177A S538A
• GenBank ID: NM_008904
• Entrez Gene: Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry