pcDNA Flag PGC1alpha S570A
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15988||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namePGC1 alpha
SpeciesM. musculus (mouse)
Insert Size (bp)3000
Entrez GenePPARGC1A (a.k.a. LEM6, PGC-1(alpha), PGC-1alpha, PGC-1v, PGC1, PGC1A, PPARGC1)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA Flag PGC1alpha S570A was a gift from Morris Birnbaum (Addgene plasmid # 15988 ; http://n2t.net/addgene:15988 ; RRID:Addgene_15988)
For your References section:Akt/PKB regulates hepatic metabolism by directly inhibiting PGC-1alpha transcription coactivator. Li X, Monks B, Ge Q, Birnbaum MJ. Nature. 2007 Jun 21. 447(7147):1012-6. 10.1038/nature05861 PubMed 17554339