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GFP-PGC1
(Plasmid #4)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 4 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGC-1 alpha
  • Alt name
    PGC1
  • Alt name
    PPAR gamma coactivator 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • GenBank ID
    NM_008904
  • Entrez Gene
    Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-PGC1 was a gift from Bruce Spiegelman (Addgene plasmid # 4 ; http://n2t.net/addgene:4 ; RRID:Addgene_4)
  • For your References section:

    A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis. Puigserver P, Wu Z, Park CW, Graves R, Wright M, Spiegelman BM. Cell 1998 Mar 20;92(6):829-39. 10.1016/S0092-8674(00)81410-5 PubMed 9529258