268,891 results

1. amajLime chromoprotein

   Plasmid

   #117843

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amajLime
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBHM2403

   Plasmid

   #171687

   Purpose

   pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   CnoCAS9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF1

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

   Plasmid

   #201916

   Purpose

   Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-Cas9 v2
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and Human U6

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pInt

   Plasmid

   #18941

   Depositor

   Michele Calos

   Article

   Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   under lacZ promoter

   Promoter

   Available since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pInt

   Plasmid

   #18941

   Depositor

   Michele Calos

   Article

   Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   under lacZ promoter

   Promoter

   Available since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJR89

   Plasmid

   #140096

   Purpose

   sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJR89

   Plasmid

   #140096

   Purpose

   sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHTN-HaloTag_RPA2:1-270

   Plasmid

   #211013

   Purpose

   Mammalian expression of full-length human RPA2. N-terminal HaloTag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   RPA2:M1-E270 (RPA2 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pNIC28-Bsa4

   Plasmid

   #26103

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNIC28-Bsa4

    Plasmid

    #26103

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-CMV-puro-2A-TEVp

    Plasmid

    #99610

    Purpose

    Lentivirus expressing puromycin resistance gene and TEV protease

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    Puromycin resistance (PAC gene)
    TEV protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-CMV-puro-2A-TEVp

    Plasmid

    #99610

    Purpose

    Lentivirus expressing puromycin resistance gene and TEV protease

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    Puromycin resistance (PAC gene)
    TEV protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hCRISPRa-v2, Membrane Proteins (h6), top 5 sgRNAs/gene

    Pooled Library

    #83985

    Purpose

    Human CRISPRa Pooled Library targeting membrane proteins

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pPEG

    Plasmid

    #162466

    Purpose

    Entry vector for gRNA insertion in plant prime editing expression vector

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Type

    Empty backbone

    Use

    Entry vector for grna

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-Fab_8D3_2_H-H6

    Plasmid

    #176075

    Purpose

    Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    His6 tagged heavy chain of Fab_8D3_2
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-Fab_8D3_2_L

    Plasmid

    #176076

    Purpose

    Express light chain of Fab_8D3_2 in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    Light chain of Fab_8D3_2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRIP-CMV-Puro-2A

    Plasmid

    #102611

    Purpose

    Control lentivector expressing Puro resistance and gene of interest from CMV promoter

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTRIP-CMV-Puro-2A

    Plasmid

    #102611

    Purpose

    Control lentivector expressing Puro resistance and gene of interest from CMV promoter

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmtb-t7-alpha-bungarotoxin

    Plasmid

    #69542

    Purpose

    For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

    Depositor

    Sean Megason

    Article

    Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

    Insert

    alpha-bungarotoxin
    

    Use

    Tol2, beta-actin promoter, sp6,t7 for zebrafish

    Tags

    Expression

    Mutation

    Promoter

    beta-actin, sp6, t7

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmtb-t7-alpha-bungarotoxin

    Plasmid

    #69542

    Purpose

    For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

    Depositor

    Sean Megason

    Article

    Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

    Insert

    alpha-bungarotoxin
    

    Use

    Tol2, beta-actin promoter, sp6,t7 for zebrafish

    Tags

    Expression

    Mutation

    Promoter

    beta-actin, sp6, t7

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge