167,514 results

1. GADD45 WT-luc

   Plasmid

   #8356

   Depositor

   Michael Greenberg

   Article

   Tran et al Science 2002 Apr 19;296(5567):530-4.

   Insert

   GADD45 promoter WT (GADD45A Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP13897 - pAAV-AiE0671m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3897)

   Plasmid

   #224038

   Purpose

   AiE0671m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   NA

   Promoter

   minBG

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-IKKbeta (S177E,S181E)-pcw107

   Plasmid

   #64609

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   IKBKB
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   S177E, S181E

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

   Plasmid

   #71294

   Purpose

   Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Tags

   GNSTM glycosylation motif, HA, Lamp2 signal pepti…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-CMV-Flag-GFP_IDG-K

   Plasmid

   #135275

   Purpose

   Gateway destination clone of GFP (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

   Depositor

   Ben Major

   Insert

   Flag-GFP
   

   Use

   Lentiviral; Gateway destination

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-MARK3-CR

   Plasmid

   #229422

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   MARK3-CR (MARK3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   silent mutation CRISPR resistant

   Promoter

   EFS

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBSKΔB- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo

   Plasmid

   #62346

   Purpose

   Expresses rtTA2sM2 and tTSkid in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

   Insert

   rtTA2sM2-IRES-tTSkid
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3990 pET ubcH8

   Plasmid

   #8650

   Depositor

   Peter Howley

   Article

   Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

   Insert

   UbcH8 (UBE2L6 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pCM184-ParB2::eGFP

   Plasmid

   #87252

   Purpose

   Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   ParB2
   

   Tags

   EGFP

   Expression

   Yeast

   Promoter

   pBAD/tetO

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV pCAG-FLEX-tdTomato-WPRE

    Plasmid

    #51503

    Purpose

    Can be used to generate AAV virus that will express tdTomato in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    tdTomato
    

    Use

    AAV

    Promoter

    pCAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-Myc-Parkin

    Plasmid

    #17612

    Depositor

    Ted Dawson

    Article

    Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

    Insert

    Parkin (PRKN Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 IKKE K38A flag

    Plasmid

    #26202

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon K38A (IKBKE Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Catalytic Lysine changed to Alanine (K38A)

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1-YY1

    Plasmid

    #169018

    Purpose

    Expression of YY1 driven by CMV promoter

    Depositor

    Claes Wadelius

    Article

    Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

    Insert

    human YY1 (YY1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF.myc.ER-E2-Crimson

    Plasmid

    #38770

    Purpose

    In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

    Depositor

    Benjamin Glick

    Article

    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

    Insert

    E2-Crimson
    

    Tags

    ER retention signal (KDEL), ER signal sequence (M…

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. KW57_HART_RAC1

    Plasmid

    #207399

    Purpose

    Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR

    Depositor

    Stephen Floor

    Article

    Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIS1-Eef25UTR-renilla

    Plasmid

    #38235

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Eef2 5'UTR / Renilla (Eef2 Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Eef2 5' UTR fused to renilla ORF

    Promoter

    Endogenous Eef2

    Available Since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Syn-ChrimsonR-GFP

    Plasmid

    #58806

    Purpose

    AAV expression of ChrimsonR-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    Syn

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINB-AMSH*

    Plasmid

    #66712

    Purpose

    Expresses activated AMSH in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Inserts

    hbp (Stam2 Mouse)
    AMSH (STAMBP Human)
    

    Tags

    His-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

19. B-gTEMP/pcDNA3

    Plasmid

    #188447

    Purpose

    B-gTEMP for mammalian expression

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJC005.em

    Plasmid

    #182738

    Purpose

    CRISPR-Cas12a genome editing of E. faecium

    Depositor

    James Collins

    Article

    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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