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pFA6a-His3MX6
(Plasmid #41596)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41596 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 2300
  • Vector type
    Yeast genomic targeting
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    His3
  • Alt name
    k-HIS3
  • Alt name
    SKHIS3
  • Species
    S. kluyveri
  • Insert Size (bp)
    1403
  • Tags / Fusion Proteins
    • A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
    • A. gossypii translation elongation factor 1a gene terminator (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer Sp6_primer
  • 3′ sequencing primer T7_promoter
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Please note that the associated publication contains important primer information for the intended application of this plasmid, but refers to a version of the plasmid containing the S. pombe HIS5 gene while this plasmid contains the S. kluyveri HIS3 gene. The generation of both plasmids is described in Wach, A., Brachat, A., Alberti-Segui, C., Rebischung, C. and Philippsen, P. (1997). Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae. Yeast 13, 1065–1075. Pubmed ID: 9290211.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-His3MX6 was a gift from John Pringle (Addgene plasmid # 41596 ; http://n2t.net/addgene:41596 ; RRID:Addgene_41596)
  • For your References section:

    Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61. 10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U PubMed 9717241