Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pFA6a-kanMX6
(Plasmid #39296)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39296 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 2300
  • Vector type
    Yeast genomic targeting
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KanR
  • Alt name
    kanamycin resistance cassette
  • Insert Size (bp)
    1600
  • Tags / Fusion Proteins
    • A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
    • A. gossypii translation elongation factor 1a gene terminator (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer Sp6_primer
  • 3′ sequencing primer T7_promoter
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-kanMX6 was a gift from Jurg Bahler & John Pringle (Addgene plasmid # 39296 ; http://n2t.net/addgene:39296 ; RRID:Addgene_39296)
  • For your References section:

    Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51. 10.1002/(SICI)1097-0061(199807)14:10<943::AID-YEA292>3.0.CO;2-Y PubMed 9717240