|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35122||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneTransformed E.coli cells are resistant to ampicillin. The plasmid contains the hph gene from Klebsiella pneumoniae encoding hygromycin B phosphotransferase and confers resistance to the antibiotic hygromycin B of transformed yeasts. Other plasmids conferring hygromycin B resistance: pAG26, pAG34. Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants
Vector typeYeast replicating
Selectable markersHygromycin B
Growth in Bacteria
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAG32 was a gift from John McCusker (Addgene plasmid # 35122 ; http://n2t.net/addgene:35122 ; RRID:Addgene_35122)
For your References section:Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Goldstein AL, McCusker JH. Yeast. 1999 Oct;15(14):1541-53. 10.1002/(SICI)1097-0061(199910)15:14<1541::AID-YEA476>3.0.CO;2-K PubMed 10514571