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pAG35
(Plasmid #35123)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35123 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6
  • Modifications to backbone
    Transformed E.coli cells are resistant to ampicillin. The plasmids contains the nat1 gene from Streptomyces noursei encoding nourseothricin N-acetyl-transferase and confers resistance to the antibiotic nourseothricin of transformed yeasts. In contrast to pAG25 this plasmid contains direct repeats of 500 bp from Aschbya gossypii flanking the insert. Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants. Provides the possiblity for a marker rescue by homologous recombination at the direct repeats
  • Vector type
    Yeast replicating
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    natMX3
  • Species
    Streptomyces noursei

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG35 was a gift from John McCusker (Addgene plasmid # 35123 ; http://n2t.net/addgene:35123 ; RRID:Addgene_35123)
  • For your References section:

    Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Goldstein AL, McCusker JH. Yeast. 1999 Oct;15(14):1541-53. 10.1002/(SICI)1097-0061(199910)15:14<1541::AID-YEA476>3.0.CO;2-K PubMed 10514571