Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24288||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7952
Vector typeInsect Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameAlpha-Tubulin at 84B
SpeciesD. melanogaster (fly)
Insert Size (bp)1353
Entrez GenealphaTub84B (a.k.a. Dmel_CG1913, 1t, ALPHA 84C, Alpha-Tubulin84B, BEST:LD32507, CG1913, DM1alpha, DTA1, Dmel\CG1913, T, TBA1_DROME, Talpha1, Tub, Tub 84B, Tub1C, Tuba84B, Tubalpha1, a-tub84B, a1t, aTub84B, alpha-Tub, alpha-Tub84B, alpha-tub, alpha-tub84B, alpha-tubulin, alpha-tubulin 84B, alpha1, alpha1t, alpha1tub, alpha1tub84B, alpha84B, alphaT, alphaTUB, alphaTUB84B, alphaTub, alphaTub1, alphaTub84, alphat-1, alphatub, alphatub84B, anon-EST:Liang-1.59, anon-EST:Liang-2.30, chr3R:2914276..2914416, clone 1.59, clone 2.30, l(3)84Bd, l(3)g3, ms(3)nc33, nc33, tub, tubA84B, tubalpha1, tubulin)
/ Fusion Protein
- mCherry (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site AttR1 (unknown if destroyed)
- 3′ cloning site AttR2 (unknown if destroyed)
- 5′ sequencing primer RFP Fwd (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Tub84b cDNA was cloned, inserted into pENTR-D-Topo and the insert was transferred into pAC-mCherry-W using LR cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAc-mCh-Tub was a gift from Ron Vale (Addgene plasmid # 24288 ; http://n2t.net/addgene:24288 ; RRID:Addgene_24288)
For your References section:Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells. Vale RD, Spudich JA, Griffis ER. J Cell Biol. 2009 Sep 7. 186(5):727-38. 10.1083/jcb.200902083 PubMed 19720876