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Ac5-STABLE2-neo
(Plasmid #32426)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32426 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Ac5/V5-HIS
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Insect Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Flag-mCherry-T2A-GFP-T2A-neo
  • Promoter Actin5C

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer AC5_PRIMER
  • 3′ sequencing primer EBVrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For multicistronic expression in insect cells.
FLAG-mCherry, GFP, or Neo can be removed or replaced.
N-terminal or C-terminal fusions to either FLAG-mCherry or GFP are possible.
Note: mCherry DNA sequence is modified from original Tsien sequence (AA sequence is unchanged). AA sequence of T2A peptides is identical, but DNA sequence differs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ac5-STABLE2-neo was a gift from Rosa Barrio & James Sutherland (Addgene plasmid # 32426 ; http://n2t.net/addgene:32426 ; RRID:Addgene_32426)
  • For your References section:

    Generation of stable Drosophila cell lines using multicistronic vectors . González, Monika; Martín-Ruíz, Itziar; Jiménez, Silvia; Pirone, Lucia; Barrio, Rosa; & Sutherland, James D. . Scientific Reports 1, Article number: 75 10.1038/srep00075