|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32426||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeInsect Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth Strain(s)XL10 Gold
- Promoter Actin5C
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer AC5_PRIMER
- 3′ sequencing primer EBVrev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
For multicistronic expression in insect cells.
FLAG-mCherry, GFP, or Neo can be removed or replaced.
N-terminal or C-terminal fusions to either FLAG-mCherry or GFP are possible.
Note: mCherry DNA sequence is modified from original Tsien sequence (AA sequence is unchanged). AA sequence of T2A peptides is identical, but DNA sequence differs.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Ac5-STABLE2-neo was a gift from Rosa Barrio & James Sutherland (Addgene plasmid # 32426 ; http://n2t.net/addgene:32426 ; RRID:Addgene_32426)
For your References section:Generation of stable Drosophila cell lines using multicistronic vectors . González, Monika; Martín-Ruíz, Itziar; Jiménez, Silvia; Pirone, Lucia; Barrio, Rosa; & Sutherland, James D. . Scientific Reports 1, Article number: 75 10.1038/srep00075