pMT-puro
(Plasmid #17923)

• Purpose: (Empty Backbone) Insect expression vector
• Depositing Lab: David Sabatini
• Publication: Unpublished plasmid (unpublished)

Backbone

• Vector backbone: pMT-puro
• Vector type: Insect Expression ; Drosophila metallothionein gene promoter drives expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: MT forward

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Test
• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insect expression vector.

This was the empty vector used in http://www.addgene.org/pubmed/17589500

This is NOT the vector by the same name described in the Castellino lab's 2008 Cytotechnology paper.

Notes from the Sabatini lab on using this plasmid:
They did a puromycin kill curve and ended up using 2 ug/mL of puromycin for selection for S2R cells. They used 5 mM CuSO4 to induce expression of their gene 24 hours before performing the IF experiments. The metallothionein promoter is described in this link: https://www.ncbi.nlm.nih.gov/pubmed/3125519

Information for Cloning Grade DNA (Catalog # 17923-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here