pMT-puro
(Plasmid #17923)

• Purpose: (Empty Backbone) Insect expression vector
• Depositing Lab: David Sabatini
• Publication: Unpublished plasmid (unpublished)

Backbone

• Vector backbone: pMT-puro
• Vector type: Insect Expression ; Drosophila metallothionein gene promoter drives expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: MT forward

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Test
• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insect expression vector.

This was the empty vector used in http://www.addgene.org/pubmed/17589500

This is NOT the vector by the same name described in the Castellino lab's 2008 Cytotechnology paper.

Notes from the Sabatini lab on using this plasmid:
They did a puromycin kill curve and ended up using 2 ug/mL of puromycin for selection for S2R cells. They used 5 mM CuSO4 to induce expression of their gene 24 hours before performing the IF experiments. The metallothionein promoter is described in this link: https://www.ncbi.nlm.nih.gov/pubmed/3125519

Information for Cloning Grade DNA (Catalog # 17923-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here