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pActPL-Gal4AD
(Plasmid #15303)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15303 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gal4AD-ZIP+
  • Alt name
    GAL4AD-EE12RR345L
  • Alt name
    GAL4
  • Insert Size (bp)
    670
  • Entrez Gene
    GAL4 (a.k.a. YPL248C, GAL81)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer Gal4-AD
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The heterodimerizing leucine zipper is based on the chicken B-Zip family member Vitellogenin Binding Protein. The EE12RR345L (Zip+) domain was generated from synthetic oligonucleotides with codon usage optimized for Drosophila expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pActPL-Gal4AD was a gift from Benjamin White (Addgene plasmid # 15303 ; http://n2t.net/addgene:15303 ; RRID:Addgene_15303)
  • For your References section:

    Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. Luan H, Peabody NC, Vinson CR, White BH. Neuron. 2006 Nov 9. 52(3):425-36. 10.1016/j.neuron.2006.08.028 PubMed 17088209